In vitro synthesis of cell wall polysaccharides and their characterization

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In vitro synthesis of cellulose was conducted with several cellulose-producing organisms. When green algae Micrasterias crux-melientes used, β1→3-D-glucan was the major product as well as plant cultured cells used. We reconfirmed by electron diffraction, X-ray diffraction and FTIR that cellulose II was synthesized in vitro when Gluconacetobacter xylinus (vinegar-producing bacterium) used, as shown previously. Noticeably, it was shown by MALDI-ToF MS for the first time that this cellulose was not oligomer like many of artificially synthesized cellulose, but probably polymer. It is thus concluded that solubilization by detergent does not damage seriously the cellulose synthesizing activity of G. xylinus.