Start-up study of functional analysis of cellulose synthase

纤维素合酶产生纤维素的机制尚不清楚，因为这种酶很难研究，而且很少对其活性进行直接分析。为了对纤维素合酶进行直接分析，本项目准备了三种材料：(1)大肠杆菌表达系统的纤维素合酶复合体重组蛋白；(2)体外和大肠杆菌中c-di-GMP合成以充分激活该酶；(3)体外纤维素合成活性的酶动力学。这些都对纤维素合酶的直接分析非常重要；特别是(i)是世界上的第一个例子，是一个杰出的研究资源。本研究为深入研究纤维素生物合成提供了重要工具。

The mechanism how cellulose synthase produces cellulose is not understood well, because this enzyme is very difficult to investigate and the enzymatic activity has been rarely analyzed in direct. In order to conduct direct analysis of cellulose synthase, three materials are prepared in this project :(i) recombinant proteins of cellulose synthase complex by E. coli expression system,(ii) c-di-GMP synthesis in vitro and in E. coli to fully activate the enzyme and(iii) enzymatic kinetics of cellulose-synthesizing activity in vitro. All of these are very important for direct analysis of cellulose synthase ; especially(i) is the first example in the world and an outstanding research resource. Thus this study has set up important tools for studying cellulose biosynthesis deeply.