Community analysis of subsurface biosphere responsible for methane cycling

负责甲烷循环的地下生物圈群落分析

• 批准号: 20244089
• 负责人: KAMAGATA Yoichi
• 金额: $ 31.03万
• 依托单位: National Institute of Advanced Industrial Science and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-20244089/
• 关键词: 天然ガス; 油田; 地下圏生物; メタン菌; メタン酸化菌; 地球化学; メタン生成古細菌; 地下圏微生物; 水溶性天然ガス; ゲノム解析; 水素・酢酸からのメタン生成; 嫌気的メタン酸化; 水素からのメタン生成

To shed light on the subsurface methane cycling microbial communities, we have analyzed the community structures, cultivated the dominant organisms, and analyzed the basic traits of isolated organisms : (1) we were successful in isolating dominant hydrogenotrophic methanogens in an association water sample in a natural gas field. The isolate was found to be distinct from know methanogens ; (2) acetate was found to be the most important intermediate in a deep subsurface oil reservoir in which syntrophic acetate oxidation occurred ; (3) anaerobic methane oxidation was detected in terrestrial subsurface samples ; (4) a syntrophic aerobic methane oxidizing community that comprised at least three members of bacteria was constructed.

为了揭示地下甲烷循环微生物群落结构，对地下甲烷循环微生物群落进行了分析，并对优势菌进行了培养，分析了分离菌的基本特征：（1）成功分离出天然气田地下甲烷循环水体中的优势产氢甲烷菌。分离物被发现是不同的从已知的产甲烷菌;（2）乙酸被发现是最重要的中间体在深地下油藏中发生互养乙酸氧化;（3）厌氧甲烷氧化在陆地地下样品中被检测到;（4）一个互养好氧甲烷氧化社区，包括至少三个成员的细菌被构建。

项目成果

Anaerobic oxidation of methane in the terrestrial subsurface environment.

陆地地下环境中甲烷的厌氧氧化。

• 发表时间: 2010
• 作者: Takeuchi M.;H.Yoshioka;Y.Seo;S.Tanabe;H.Tamaki ;Y.Kamagata;H.A.Takahashi;S.Igari;D.Mayumi;S.Sakata
• 通讯作者: S.Sakata

Methanogenic pathway in a high-temperature petroleum reservoir assessed by stable isotope tracer and molecular biological techniques.

通过稳定同位素示踪剂和分子生物学技术评估高温石油藏的产甲烷途径。

• 发表时间: 2010
• 作者: 眞弓大介;坂田将;坂田将;宮川喜洋;五十嵐雅之
• 通讯作者: 五十嵐雅之

Evidence for syntrophic acetate oxidation coupled to hydrogenotrophic methanogenesis in the high-temperature petroleum reservoir of Yabase oil field (Japan)

• DOI: 10.1111/j.1462-2920.2010.02338.x
• 发表时间: 2011-08-01
• 期刊: ENVIRONMENTAL MICROBIOLOGY
• 影响因子: 5.1
• 作者: Mayumi, Daisuke;Mochimaru, Hanako;Kamagata, Yoichi
• 通讯作者: Kamagata, Yoichi

Ecology of methanogenic archaea

产甲烷古菌生态学

• 发表时间: 2009
• 作者: Katsuki;K.;丸山茂徳;鎌形洋一
• 通讯作者: 鎌形洋一

Apparent richness and community composition of Bacteria and Archaea in geothermal springs

• DOI: 10.3354/ame01333
• 发表时间: 2009-01-01
• 期刊: AQUATIC MICROBIAL ECOLOGY
• 影响因子: 1.4
• 作者: Kormas, Konstantinos Ar.;Tamaki, Hideyuki;Kamagata, Yoichi
• 通讯作者: Kamagata, Yoichi