Community analysis of subsurface biosphere responsible for methane cycling

为了阐明地下甲烷循环微生物群落，我们分析了群落结构，培养了优势菌，并分析了分离生物的基本特征：(1)从某天然气田伴生水样中成功分离到优势产氢产甲烷菌。该菌株与已知的产甲烷菌不同；(2)乙酸盐是深层地下油藏中最重要的中间产物，在该油藏中发生了乙酸酯氧化；(3)在陆地地下样品中检测到厌氧甲烷氧化；(4)构建了一个至少由3个细菌组成的合养好氧甲烷氧化群落。

To shed light on the subsurface methane cycling microbial communities, we have analyzed the community structures, cultivated the dominant organisms, and analyzed the basic traits of isolated organisms : (1) we were successful in isolating dominant hydrogenotrophic methanogens in an association water sample in a natural gas field. The isolate was found to be distinct from know methanogens ; (2) acetate was found to be the most important intermediate in a deep subsurface oil reservoir in which syntrophic acetate oxidation occurred ; (3) anaerobic methane oxidation was detected in terrestrial subsurface samples ; (4) a syntrophic aerobic methane oxidizing community that comprised at least three members of bacteria was constructed.