The construction for recycle of fluorine resource with the complex method

我们发现分离出了能降解含氟物质的菌株，8株放线菌表现出可降解性，二氟乙酸乙酯（DFAc）被细菌积累，先放出二氟乙酸，再放出氟离子。此外，13株放线菌表现出对氟苯和/或苯三氟的降解能力。在批培养中，菌株在含氟材料上生长导致氟离子的释放。在氟离子存在下，利用s -腺苷- l-蛋氨酸合成酶（MetK）、氟酶和甲基硫代腺苷核苷酶（MtnN），建立了ATP和l-蛋氨酸一锅三步连续酶法合成5-氟-5-脱氧d -核糖（5-FDR）的方法。特别是，为了氟化学的可持续发展，我们建立了利用BF4离子液体产生的氟离子和/或在一锅合成过程中将BTF生物降解为5-FDR的方法。此外，我们还确定了BTF生物降解产生的氟离子再转化为氟化钙。

We have found the isolation of bacterial strains capable of degrading fluorinated materials, 8 strains of Actinobacteria exhibited degradability of ethyl difluoroacetate(DFAc) was accumulated by bacteria, giving difluoroacetic acid and then fluoride ion. Further, 13 strains of Actinobacteria exhibited degradability of fluorobenzene and/or benzotrifluoride. In batch culture, growth of strains on fluorinated materials led to the release of fluoride ion. We have established the one-pot three-step continuous enzymatic synthesis of 5-fluoro-5-deoxy-D-ribose(5-FDR) from ATP and L-methionine using S-adenosyl-L-methionine synthase(MetK), fluorinase and methylthioadenosinenucleosidase(MtnN) in the presence of fluoride ion. Especially, for the purpose of thesustainable development of fluorine chemistry, we have established the reuse of fluoride ion generated from BF4 ionic liquids and/or the biodegradation of BTF in the one-pot synthetic process to 5-FDR. Further, we have established the retransformation of fluoride ion generated from biodegradation of BTF to calcium fluoride.