Innovation of antibody enzymes and their application to produce the materials

本文研究了在抗体醛缩酶38C2 (Aldrich no 48157-2)-离子液体（[bmim][PF_6]）体系中取代苯甲醛与丙酮衍生物的醛缩反应。室温搅拌14天后，用乙醚提取原料和产物，回收离子液体和抗体醛缩酶32C2。结果表明，在该体系中，羟基丙酮与4-或3-（三氟甲基）苯甲醛反应生成3,4-二羟基-4-（4-或3-三氟甲基）苯丁醇-2- 1。而丙酮、甲基乙基酮、甲氧基丙酮、氟丙酮和氯丙酮均未在该体系中析出。此外，在脂肪族和/或α， β-不饱和醛的情况下，我们不能进行醛醇反应。对回收的ab32c2离子液体体系进行连续重复使用，在相同的反应中，3-和4-（三氟甲基）苯甲醛的产量都高于第一次循环。在第三和第四个循环中，从第二个循环中回收的该系统可以在相同的反应中产生相同的醇。下一步，我在离子液体[emim][OTf]-醛缩酶抗体38C2体系中检测了羟丙酮与2-三氟甲基丙烯酸苯乙酯的Michael加成反应。总之，这种环境友好型溶剂和抗体催化剂组合的成功应用令人兴奋，并可能为开发其他重要有机反应的类似反应体系打开大门。

I examined tha aldol reaction of substituted benzaldehyde and acetone derivative in the antibody aldolase 38C2 (Aldrich no 48157-2)-ionic liquid ([bmim][PF_6]) system. After stirring for 14 days at room temperature, starting materials and products were extracted with diethyl ether, and ionic liquid and antibody aldolase 32C2 were recovered. From the results, we have found that the reaction of hydroxyacetone with 4-or 3-(trifluoromethyl) benzaldehyde in this system was proceeded to produce the 3,4-dihydroxy-4-(4-or 3-trifluoromethyl) phenylbutan-2-One. However, acetone, methyl ethyl ketone, methoxyacetone, fluoroacetone and chloroacetone were not leacted in this system. Moreover, in the cases of aliphatic and/or α, β-unsaturated aldehydes, we did not able to proceed the aldol reaction. Successive reuse of the recovered Ab32C2-ionic liquid system and in the same reaction yielded amounts of product as higher than the first cycle in the both cases of 3-and 4-(trifluoromethyl) bezaldehyde. In the third and fourth cycles, reuse of this system recovered from the second cycle is possible to produce the same alcohol in the same reaction.In the next step, I examined the Michael addition reaction of hydroxyacetone to phenetyl 2-trifluoromethyacrylate in the ionic liquid [emim][OTf]-aldolase antibody 38C2 system.In conclusion, the successful application of this combination of environmentally friendly solvent and antibody catalyst is exciting and is likely to open the door to the development of similar reaction systems for other important organic reactions.