Quantitative analysis of protein-protein interaction inside animal-derived cells

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Construction of quantitative analysis system for protein-protein interaction inside animal-derived cells was attempted with controlling those protein expression levels using peptide nucleic acid (PNA)-peptide conjugates. We successfully achieved three indispensable basic elements for the system as follows: (1) construction of novel protein-expression control system, (2) construction of detection system for self-assembly reaction as a first step in establishing the analysis system of protein-protein interaction inside cells, (3) construction of cell array system for high-throughput analysis.