Functional analysis of CD9 molecule and oocyte microvilli in fertilization by novel non-invasive imaging method.

采用新型非侵入性成像方法分析受精过程中 CD9 分子和卵母细胞微绒毛的功能。

• 批准号: 21870021
• 负责人: SATOUH Yuhkoh
• 金额: $ 1.76万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21870021/
• 关键词: 遺伝子操作動物; 実験動物; 受精; 微絨毛; 膜融合

In this study, I focus on the function of CD9 molecule and egg microvilli in mammalian fertilization. For that, I set two goals as 1) Analysis of fertilization ability of microvilli less mutant egg, and 2) Visualization of egg microvilli by using novel non-invasive imaging method. As a result until 2011, 1) I have obtained the data which strongly suggesting that the eggs without two of essential genes for constructing microvilli don't have fertilizing ability in vivo. And 2) I have established the imaging system which enable us to visualize egg microvilli in living form. By using the imaging system, I additively succeeded in visualizing the process of sperm-egg fusion in real-time scale for the first time.

本论文主要研究CD9分子和卵微绒毛在哺乳动物受精过程中的作用。为此，我设定了两个目标：1）分析微绒毛缺失突变卵子的受精能力，2）使用新的非侵入性成像方法可视化卵子微绒毛。结果，直到2011年，1）我已经获得的数据强烈表明，没有两个构建微绒毛的必需基因的卵子在体内不具有可繁殖性。2）建立了活体卵微绒毛成像系统。通过使用该成像系统，我首次成功地将精卵融合过程实时可视化。

项目成果

Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization

• DOI: 10.1073/pnas.1018202108
• 发表时间: 2011-03-22
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Jin, Mayuko;Fujiwara, Eiji;Hirohashi, Noritaka
• 通讯作者: Hirohashi, Noritaka

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Sperm equatorial segment protein 1, SPESP1, is required for fully fertile sperm in mouse

• DOI: 10.1242/jcs.067363
• 发表时间: 2010-05-01
• 期刊: JOURNAL OF CELL SCIENCE
• 影响因子: 4
• 作者: Fujihara, Yoshitaka;Murakami, Masao;Okabe, Masaru
• 通讯作者: Okabe, Masaru

Calsperin Is a Testis-specific Chaperone Required for Sperm Fertility

• DOI: 10.1074/jbc.m110.140152
• 发表时间: 2011-02-18
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Ikawa, Masahito;Tokuhiro, Keizo;Okabe, Masaru
• 通讯作者: Okabe, Masaru