Visuallization of the spatio-temporal activities of Rab GTPases forming Rab cascades

在酿酒酵母菌中提出了Rab GTPase级联调控ER-to-Golgi分泌途径。参与ER到顺式高尔基转运的Ypt1p位于Ypt31/32p的上游，介导后高尔基囊泡从反式高尔基体出芽。Ypt31/32p作用于Sec4p的上游，Sec4p是与高尔基后衍生的分泌囊泡相关的Rab GTPase。在这项研究中，我开发了这些Rab gtpase的分子内FRET探针，以可视化活酵母细胞中的时空活性。

Rab GTPase cascade in the regulation of ER-to-Golgi step of the secretory pathway was proposed in Saccaromyces cerevisiae. Ypt1p involved to ER to cis-Golgi transport acts upstream of the Ypt31/32p, mediating the budding of post-Golgi vesicles from the trans-Golgi. Ypt31/32p acts upstream of Sec4p, the Rab GTPase associated with post Golgi derived secretory vesicles. In this study, I developed the intra molecular FRET probes of these Rab GTPases to visualize the spacio-temporal activities in the living yeast cells.