Peptidome analysis focusing on C-terminal amidation : Identification and functional analysis of novel bioactive peptides.

专注于 C 末端酰胺化的肽组分析：新型生物活性肽的鉴定和功能分析。

• 批准号: 21870055
• 负责人: WAKABAYASHI Masaki
• 金额: $ 1.76万
• 依托单位: National Cardiovascular Center Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21870055/
• 关键词: 生理活性ペプチド; ペプチドーム解析; C末端アミド化; 抗体アフィニティーカラム

Bioactive peptides play crucial roles in complex homeostasis regulatory systems in living organisms. Thus, it is essential to identify novel bioactive peptides and analyze their physiological functions to elucidate the homeostasis regulatory systems. In this study, we tried to isolate new bioactive peptides using their frequently observed structural characteristic, C-terminal amidation, as a hallmark. We developed a method for condensation of C-terminally amidated peptides from naturally occurring peptide mixtures, though a novel bioactive peptide was not identified.

生物活性肽在生物体内复杂的稳态调节系统中起着至关重要的作用。因此，鉴定新的生物活性肽并分析其生理功能是阐明体内平衡调控系统的必要条件。在这项研究中，我们试图利用其经常观察到的结构特征，c端酰胺作为标志来分离新的生物活性肽。我们开发了一种从天然肽混合物中缩合c端修饰肽的方法，尽管尚未鉴定出新的生物活性肽。

项目成果

Global Identification of peptide processing sites implicated in the regulated secretory pathway

与调节的分泌途径有关的肽加工位点的全局鉴定

• 发表时间: 2010
• 作者: Masaki Wakabayashi;Kazuki Sasaki;Tsukasa Osaki;Yoshinori Satomi;Toshifumi Takao;Naoto Minamino
• 通讯作者: Naoto Minamino