Peptidome analysis focusing on C-terminal amidation : Identification and functional analysis of novel bioactive peptides.

生物活性肽在生物体内复杂的稳态调节系统中起着至关重要的作用。因此，鉴定新的生物活性肽并分析其生理功能是阐明体内平衡调控系统的必要条件。在这项研究中，我们试图利用其经常观察到的结构特征，c端酰胺作为标志来分离新的生物活性肽。我们开发了一种从天然肽混合物中缩合c端修饰肽的方法，尽管尚未鉴定出新的生物活性肽。

Bioactive peptides play crucial roles in complex homeostasis regulatory systems in living organisms. Thus, it is essential to identify novel bioactive peptides and analyze their physiological functions to elucidate the homeostasis regulatory systems. In this study, we tried to isolate new bioactive peptides using their frequently observed structural characteristic, C-terminal amidation, as a hallmark. We developed a method for condensation of C-terminally amidated peptides from naturally occurring peptide mixtures, though a novel bioactive peptide was not identified.