Study of a new transgenic technique in coenocytic green algae utilizing their protoplast regeneration ability

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Reporter mRNA encoding Renilla luciferase was introduced into the sub-protoplasts, which were regenerated by squeezing out from the coenocytic green alga Bryopsis plumosa, using conventional transgenic methods with polyethyleneglycol and cationic lipid or a new method of which the reporter mRNA was added into the squeezing medium. The luciferase activities were detected in those sub-protoplasts introduced by the new method as well as by the conventional methods. These results indicated that a new easy transgenic technique in coenocytic green algae could be established.