Study of a new transgenic technique in coenocytic green algae utilizing their protoplast regeneration ability

利用共核绿藻原生质体再生能力的新转基因技术研究

• 批准号: 21880029
• 负责人: HIRAYAMA Makoto
• 金额: $ 1.68万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21880029/
• 关键词: 遺伝子改変技術; 海藻; 細胞再構築現象; ハネモ; 遺伝子改変

Reporter mRNA encoding Renilla luciferase was introduced into the sub-protoplasts, which were regenerated by squeezing out from the coenocytic green alga Bryopsis plumosa, using conventional transgenic methods with polyethyleneglycol and cationic lipid or a new method of which the reporter mRNA was added into the squeezing medium. The luciferase activities were detected in those sub-protoplasts introduced by the new method as well as by the conventional methods. These results indicated that a new easy transgenic technique in coenocytic green algae could be established.

使用聚乙二醇和阳离子脂质的传统转基因方法或将报告基因mRNA添加到挤压培养基中的新方法，将编码海肾荧光素酶的报告基因mRNA引入到亚原生质体中，该亚原生质体是通过从同种细胞绿藻羽状羽藻中挤出而再生的。在通过新方法和常规方法引入的亚原生质体中检测到荧光素酶活性。这些结果表明可以在共核绿藻中建立一种新的简单转基因技术。

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