Analysis of leukemia stem cell regulation by leukemia oncogene Evi-1

白血病癌基因Evi-1对白血病干细胞的调控分析

• 批准号: 21890055
• 负责人: SATO Tomohiko
• 金额: $ 1.66万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21890055/
• 关键词: 癌; 白血病; 幹細胞

We have recently generated Evi-1-GFP knock-in mice, which can trace Evi1 mRNA level in vivo, and we revealed that Evi-1 is highly expressed at both fetal and adult hematopoietic stem cells (HSCs). In addition, we also clarified that Evi-1 positive murine HSC (KSL) function as long-term HSC by bone marrow transplantation assay, and that Evi-1 can mark long-term HSC which is the most immature hematopoietic cells. (These data are now on submission) Next, for the analysis of leukemia stem cell regulation by Evi-1, we made chronic myeloid leukemia (CML) mouse of Evi-1-GFP knockin origin by using BCR-ABL oncogene. We revealed that Evi-1 positive KSL cells had highest Evi-1 expression in these model mice which implied that Evi-1 could regulate CML stem cells. Our original analysis of leukemia stem cell is still ongoing for the purpose of establishing specific therapy of CML stem cells.

我们最近已经产生了Evi-1-GFP敲入小鼠，它可以跟踪Evi 1 mRNA水平在体内，我们发现Evi-1是高度表达在胎儿和成人造血干细胞（HSC）。此外，我们还阐明了Evi-1阳性小鼠HSC（KSL）在骨髓移植实验中具有长期HSC的功能，并且Evi-1可以标记最不成熟的造血细胞--长期HSC。接下来，为了分析Evi-1对白血病干细胞的调节，我们通过使用BCR-ABL癌基因制备了Evi-1-GFP敲入来源的慢性髓性白血病（CML）小鼠。结果显示，在CML模型小鼠中，Evi-1阳性的KSL细胞中Evi-1表达最高，提示Evi-1对CML干细胞具有调控作用。我们对白血病干细胞的原始分析仍在进行中，目的是建立CML干细胞的特异性治疗。

项目成果

Evi1 expression marks long-term repopulating hematopoietic stem cells.

Evi1 表达标志着造血干细胞的长期再生。

• 发表时间: 2010
• 作者: Keisuke Kataoka;Tomohiko Sato;Akihide Yoshimi;Susumu Goyama;Shunya Arai;Yoichi Imai;Katsuyoshi Kumagai;Naoto Kubota;Takashi Kadowaki;Mineo Kurokawa
• 通讯作者: Mineo Kurokawa

Evi1 Is a Stem Cell-Specific Regulator of Self-Renewal Capacity In the Definitive Hematopoietic System

Evi1 是决定性造血系统中自我更新能力的干细胞特异性调节因子

• 发表时间: 2010
• 作者: Keisuke Kataoka;Tomohiko Sato;Akihide Yoshimi;Susumu Goyama;Takako Tsuruta;Shunya Arai;Yoichi Imai;Katsuyoshi Kumagai;Naoto Kubota;Takashi Kadowaki;Mineo Kurokawa
• 通讯作者: Mineo Kurokawa

ホームページ等。

主页等