Fundamental research project to elucidate the process in which bone marrow side population (SP) cells differentiate into muscle fibers

阐明骨髓侧群 (SP) 细胞分化为肌纤维过程的基础研究项目

• 批准号: 21890259
• 负责人: IWANUMA Osamu
• 金额: $ 1.46万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21890259/
• 关键词: 骨髄SP細胞; 筋線維; 筋分化; FACS; 成長因子; 筋特異的転写調節因子; 分化; TGF-β1; 免疫染色; 筋芽細胞; 共培養; 筋管様細胞

The decrease in myofunction is a well known degenerative change with age. To elucidate the mechanisms of myofunctional reactivation, we investigated specific proteins that promote the differentiation of bone marrow side population cells, which influence muscle development. Bone marrow side population cells were cultivated in low serum media, to which various combinations of growth factors were added. As factors involved in myofunctional reactivation, TGF-β1 and IGF-1 influenced the early and subsequent stages of differentiation, respectively.

肌功能的下降是众所周知的随年龄增长的退行性变化。为了阐明肌肉功能重新激活的机制，我们研究了促进骨髓侧群体细胞分化的特定蛋白质，这些蛋白质影响肌肉发育。骨髓侧群细胞在低血清中培养，加入不同的生长因子组合。转化生长因子-β-1和胰岛素样生长因子-1作为参与肌功能再激活的因子，分别影响分化的早期和后续阶段。

项目成果

Expression of HGF and IGF-1 during regeneration of masseter muscle in mdx mice

• DOI: 10.1007/s10974-010-9209-6
• 发表时间: 2010-07-01
• 期刊: JOURNAL OF MUSCLE RESEARCH AND CELL MOTILITY
• 影响因子: 2.7
• 作者: Honda, Hidemitsu;Abe, Shinichi;Ide, Yoshinobu
• 通讯作者: Ide, Yoshinobu

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Effect of mechanical stretching on expressions of muscle specific transcription factor MyoD, Myf-5, myogenin and MRF4 in proliferated myoblasts

机械拉伸对增殖成肌细胞中肌肉特异性转录因子MyoD、Myf-5、肌细胞生成素和MRF4表达的影响

• 发表时间: 2009
• 期刊: Anat histol embryol 38
• 作者: Abe S;Iwanuma O;et al.(7名)
• 通讯作者: et al.(7名)

マウス骨髄由来間葉系幹細胞の筋線維への分化について

小鼠骨髓间充质干细胞分化为肌纤维

• 发表时间: 2010
• 作者: 岩沼治;岸飛鳥;菊地昭仁;阿部伸一;井出吉信
• 通讯作者: 井出吉信