Improving intravital microscopy of inflammatory cell response by active motion compensation using controlled adaptive optics (A06)

使用受控自适应光学器件通过主动运动补偿改善炎症细胞反应的活体显微镜检查 (A06)

• 批准号: 452338116
• 金额: --
• 依托单位: Institut für Analysis und Numerik
• 依托单位国家: 德国
• 项目类别: Collaborative Research Centres
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/452338116?language=en
• 关键词: Improving; intravital; microscopy; inflammatory; cell

We will advance multiphoton fluorescence microscopy by developing a novel optical module comprised of a high-speed deformable mirror that will actively compensate tissue motion during intravital imaging, for instance due to heart beat (8 Hz), breathing (3 Hz, in mm-range) or peristaltic movement of the gut in mice. To control this module in real-time, we will develop mathematical methods that track and predict tissue deformation. This will allow imaging of inflammatory processes at cellular resolution without mechanical tissue fixation.

我们将通过开发一种由高速可变形镜组成的新型光学模块来推进多光子荧光显微镜，该模块将在活体成像期间主动补偿组织运动，例如由于心跳（8 Hz），呼吸（3 Hz，mm范围）或小鼠肠道的蠕动运动。为了实时控制这个模块，我们将开发跟踪和预测组织变形的数学方法。这将允许在细胞分辨率下对炎症过程进行成像，而无需机械组织固定。