roteomic, metagenomic and genome-wide analyses of oral cavity of ovariectomized rats with psychological stress

心理应激下卵巢切除大鼠口腔的蛋白质组学、宏基因组学和全基因组分析

• 批准号: 21K21094
• 负责人: PAUDEL Durga
• 金额: $ 1.91万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-08-30 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21K21094/
• 关键词: psychological stress; salivary protein; genome wide analyses; oral cavity; post menopause; oral microbiome; salivary gland

The research has progressed as planned but with a slight change in schedule. The metagenomic study and genome-wide analysis of the oral cavity in psychological stress and ovariectomized rats have been completed. The proteomic study has been planned for the coming year.Some additional experiments were performed to investigate the matter deeper. A study to determine if psychological stress/depression-related drugs affect the oral cavity. The results showed that there was a significant alteration in oral microbiome after the tricyclic antidepressant, amitriptyline was systemically administered to rats. The results were published in an international journal.Another study was performed to determine if psychological stress affects the gene expression of salivary glands in rats. The results of the genome-wide analysis showed significant alteration in gene expression and cellular and molecular pathways in the submandibular gland of rats after psychological stress. The findings were presented at an international conference in the United States.The results of metagenomic analyses and genome-wide analysis are being analyzed and the manuscript is being prepared to submit it for publication in an international journal.

研究工作按计划进行，但进度稍有变化。完成了心理应激和去卵巢大鼠口腔的宏基因组研究和全基因组分析。这项蛋白质组学研究计划在明年进行。为了更深入地研究这个问题，又进行了一些实验。一项确定心理压力/抑郁相关药物是否影响口腔的研究。结果显示，大鼠全身给予三环抗抑郁药阿米替林后，口腔微生物组有明显改变。研究结果发表在一份国际期刊上。另一项研究是为了确定心理压力是否会影响大鼠唾液腺的基因表达。全基因组分析结果显示，心理应激后大鼠颌下腺基因表达及细胞分子通路发生显著改变。研究结果在美国的一次国际会议上公布。正在对宏基因组分析和全基因组分析的结果进行分析，并准备将手稿提交在国际期刊上发表。

项目成果

Effect of Chronic Psychological Stress on Transcriptome of Submandibular Gland

慢性心理应激对颌下腺转录组的影响

• 发表时间: 2023
• 作者: Durga Paudel;Osamu Uehara;Tetsuro Morikawa;Koki Yoshida;Yoshihiro Abiko
• 通讯作者: Yoshihiro Abiko

Advanced Research Promotion Center

先进研究促进中心

Effect of Systemic Administration of Amitriptyline on Oral Microbes in Rats

全身给药阿米替林对大鼠口腔微生物的影响

• DOI: 10.21873/invivo.12939
• 发表时间: 2022
• 期刊: In Vivo
• 影响因子: 2.3
• 作者: NINOMIYA KAZUNORI;PAUDEL DURGA;UEHARA OSAMU;MORIKAWA TETSURO;ISLAM SYED TAUFIQUL;KHURELCHULUUN ARIUNTSETSEG;ARIWANSA DEDY;YOSHIDA KOKI;MATSUOKA HIROFUMI;HAJIME HARA;KAYOKO FUKUI;NAKAMURA KENJIROU;ABIKO YOSHIHIRO
• 通讯作者: ABIKO YOSHIHIRO