Development of a new method for estimating fine root biomass from the quantity of DNA of target plant

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The practical use of a new method for estimating fine root biomass of target plant from the quantity of DNA extracted from fine root mixed with soil particles was examined. A DNA extraction method was improved to obtain the optimal level of DNA extraction efficiency for Zea mays fine root mixed with Kanuma soil, which adsorbs DNA strongly. Using the improved DNA extraction method, a regression line between the amount of extracted DNA and fine root biomass could be obtained. Moreover, the estimation error in the method did not differ among the fraction sizes of fine roots.