Development of a new method for estimating fine root biomass from the quantity of DNA of target plant

开发一种根据目标植物 DNA 数量估算细根生物量的新方法

• 批准号: 22880040
• 负责人: UGAWA Shin
• 金额: $ 1.42万
• 依托单位: Forestry and Forest Products Research Institute
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22880040/
• 关键词: 細根バイオマス量; リアルタイムPCR; DNA抽出効率; スキムミルク; アロフェン; 単回帰分析; 推定誤差

The practical use of a new method for estimating fine root biomass of target plant from the quantity of DNA extracted from fine root mixed with soil particles was examined. A DNA extraction method was improved to obtain the optimal level of DNA extraction efficiency for Zea mays fine root mixed with Kanuma soil, which adsorbs DNA strongly. Using the improved DNA extraction method, a regression line between the amount of extracted DNA and fine root biomass could be obtained. Moreover, the estimation error in the method did not differ among the fraction sizes of fine roots.

研究了一种新的从与土壤颗粒混合的细根中提取DNA的量来估计目标植物细根生物量的方法的实际应用。以玉米细根为材料，利用卡努马土对DNA的强吸附性，对DNA提取方法进行了改进，以获得最佳的DNA提取效率。采用改进的DNA提取方法，可以得到DNA提取量与细根生物量之间的回归直线。此外，该方法的估计误差并不存在差异的细根的分数大小。

项目成果

DNA定量法を用いた細根バイオマス量の測定手法の開発

DNA定量法测定细根生物量方法的开发

• 发表时间: 2012
• 作者: 鵜川信;山口宗義;三浦覚;金子真司
• 通讯作者: 金子真司