Creation of a novel therapeutic antibody for tumor metastasis-Targeting Tumor Vasculogenic Mimicry-

点击翻译按钮获取中文摘要

In this study, we had an attempt to develop a novel therapy for tumor. Recent years, it is reported that in the tumor tissue tumor cells compose luminal structure as like vessel. This structure is called as tumor vasculogenic mimicry(TVM). There are many red blood cells in a part of TVM. It is expected that TVM has an important role to metastasize via blood flow. Therefore, it expects that the inhibition of both angiogenesis and TVM can result in the inhibition of tumor growth and metastasis. Therefore, we had an attempt to create anti-TVM antibody for tumor therapy, using phage-display technique. Next, we evaluated tumor growth suppressive effect by TVM vaccine therapy for tumor bearing mice to clarify how TVM development associates with metastasis. As the result, in TVM-immune mice, tumor growth had a tendency to be suppressed, compared with non-treated mice. In addition, to evaluate TVM property as blood vessel, we measured enzyme activity which has high activity in endothelial cell. As the result, in TVM, we can't confirm the elevated activity. Thus, we clarified that TVM possess properties differing from blood vessel in spite of being similar in structure to them.