Study on the novel metabolic pathway of phosphatidylinositol to produce endogenous cannabinoid.

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GPR55 is a seven-transmembrane G-protein-cpupled receptor that has been proposed as a novel type of cannabinoid receptor. Previously, we identified lysophosphatidylinositol(LPI), in particular 2-arachidonoyl-LPI, as an agonist for GPR55.In the present study, we tried to identify PI-PLA1 and PI-PLA2 to clear the novel metabolic pathway of PI. To clear the novel function of phospholipid-metabolic enzymes, we examined the efficiency of Cell-Cell fusion of overexpressed or knockdown cells. The cell-cell fusion activity were increased in these cells. These results suggest that phospholipid-metabolic enzymes are involeved in cell-cell fusion.