In vivo functional analysis of type 2 diabetes related new genes using liver siRNA delivery technique

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First, nucleic acid such as pDNA and siRNA delivery system to the liver was developed. This mission was achieved by optimizing the density of R8 peptide which has high ability to internalize into cell and GALA peptide as pH sensitive fusogenic peptide. Second, a new candidate gene which related the progression of type 2 diabetes was screened by DNA microarray analysis. Third, in vivo functional analysis of this new gene was performed using siRNA. When knocking down for this gene in the liver of type 2 diabetes model mice, significant reduction of both blood glucose level and body weight was observed. Thus, this new gene is promising therapeutic target for type 2 diabetes as well as obesity.