Label-free intracellular dynamics investigation of carrier-free nanoparticle-based drug delivery systems

点击翻译按钮获取中文摘要

In AY2022, we focused on 1) functionalizing nanoprodrugs and 2) developed the method based on Raman spectroscopy for intracellularly label-free analysis of nanoprodrugs dynamics. The surface of SN-38 nanoprodrugs was decorated with biotin-modified liposomal membranes to enhance the internalization of cancer cells and target specific cellular compartments. For the analysis of intracellular drug dynamics based on single-cell SERS endoscopy, we successfully coated silver nanowire (AgNW) probes of the endoscopy system with ZIF-8 metal-organic framework, enabling the selective detection of molecules based on their molecular sizes and hydrophobicity. The developed method is able to detect the conversion of irinotecan prodrug into SN-38 active drug molecule (Advanced optical materials, 2023 accepted).