Surface engineering of carrier-free drug nanoparticle toward highly efficient anticancer drug delivery

无载体药物纳米颗粒的表面工程可实现高效抗癌药物递送

• 批准号: 21J13093
• 负责人: TAEMAITREE FARSAI
• 金额: $ 1.25万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-28 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-21J13093/
• 关键词: Drug delivery system; Nanoparticles; Dispersion stability; Surface modification

Surface modification strategies were carefully monitored to achieve drug nanoparticles with enhanced drug delivery efficiency (Chem. Lett. 2021, 50, 1555-1558; Mol. Crys. Liq. Crys., in press 2022). For the preparation of liposomal membrane modified drug nanoparticles, the coating on the drug nanoparticle surface was achieved by the sequential extrusion of membrane vesicle with the SN-38 drug nanoparticles model system. The modified drug nanoparticles were obtained in a slightly larger size, with narrow size distribution as confirmed by DLS. The liposomal layer on the nanoparticle’s surface was successfully confirmed by fluorescence and SEM images. In addition to the enhanced dispersion stability in the physiological environment, we have also shown that the liposomal membrane layer can be further modified to provide drug nanoparticles with specific cancer targeting and fluorescence imaging capability. The optimized protocol will be employed for the modification of drug nanoparticles with the liposomal membrane derived from natural sources such as red blood cells, white blood cells, and cancer cells.

仔细监测表面改性策略以获得具有增强的药物递送效率的药物纳米颗粒（Chem.Lett. 2021，50，1555-1558; Mol.哭泣。液态哭泣，2022年出版）。以SN-38药物纳米粒为模型体系，通过连续挤出膜囊泡的方法，实现了脂质体膜修饰药物纳米粒表面的包覆。修饰的药物纳米颗粒以稍大的尺寸获得，具有窄的尺寸分布，如DLS所证实的。通过荧光和SEM图像成功地证实了纳米颗粒表面上的脂质体层。除了在生理环境中增强的分散稳定性之外，我们还表明可以进一步修饰脂质体膜层以提供具有特异性癌症靶向和荧光成像能力的药物纳米颗粒。优化的方案将用于修饰药物纳米粒与脂质体膜衍生自天然来源，如红细胞，白色血细胞，和癌细胞。

项目成果

Fabrication of Fluorescent Nanoprobes Based on FRET And Analysis of Intracellular Behavior of Organic Nanoparticles

基于 FRET 的荧光纳米探针的制备及有机纳米粒子的细胞内行为分析

• 发表时间: 2021
• 作者: 三木佑太郎;Farsai Taemaitree;鈴木龍樹;小関良卓;Anh T.N. Dao;橋本塁人;若宮淳志;笠井均
• 通讯作者: 笠井均

Dual pH- and Thermo-Responsive Au/Silk Nanocarrier for Cancer Therapy

用于癌症治疗的双 pH 和温度响应金/丝纳米载体

• 发表时间: 2021
• 作者: Anh T. N. Dao;R. Sawamura;R. Karashimada;N. Iki;F. Taemaitree;H. Kasai
• 通讯作者: H. Kasai

Facile Fabrication of Silk Protein for a Versatile Drug Carrier

轻松制备丝蛋白作为多功能药物载体

• 发表时间: 2021
• 作者: Anh T. N. Dao;Farsai Taemaitree;Motofumi Nakamura;Ryuju Suzuki;Yoshitaka Koseki;Hitoshi Kasai
• 通讯作者: Hitoshi Kasai

有機ナノ粒子の細胞内溶解過程を追跡できる高発光性ナノ粒子の開発

开发可追踪有机纳米颗粒细胞内溶解过程的高发光纳米颗粒

• 发表时间: 2022
• 作者: 三木 佑太郎;Farsai Taemaitree;鈴木 龍樹;小関 良卓;Anh T;N. Dao;橋本 塁人;若宮 淳志;笠井 均
• 通讯作者: 笠井 均

Intracellular investigation of SN-38 prodrug nanoparticles as carrier-free anticancer drug delivery systems

SN-38 前药纳米粒子作为无载体抗癌药物递送系统的细胞内研究

• 发表时间: 2021
• 作者: Farsai Taemaitree;Beatrice Fortuni;Yoshitaka Koseki;Hiroshi Uji-i;Tomoko Inose;Hitoshi Kasai
• 通讯作者: Hitoshi Kasai