Production of 2-D nanocarbons with different bioaffinities as ROS quenching agents to improve mammalian oocyte cryopreservation outcomes

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We prepared graphene oxide (GO) according to a previous method (Hummer’s method) and slowly performed thermal reduction at 80°C up to 2 weeks to ensure a successful reduction of the functional groups to obtain reduced graphene oxide (rGO). The GO and rGO samples were structurally analyzed by FTIR, TGA, XPS and XRD. To analyze their reactive oxygen species (ROS) activity, they were analyzed by ESR. Furthermore, to understand their ability to be used in a culture medium setting, the samples were submitted to a dispersion test.According to the results of all the tests, pristine GO (oxidation level 30-35%) was selected to analyze its toxicity during oocyte in vitro maturation (IVM) in the presence of non-enzymatic antioxidants. Results suggest that the addition of GO during oocyte IVM does not negatively affect oocyte viability significantly.