Functional and structural annotation of small proteins by advanced proteomics approaches

通过先进的蛋白质组学方法对小蛋白质进行功能和结构注释

• 批准号: 453130481
• 负责人: Professor Dr. Juri Rappsilber
• 金额: --
• 依托单位: Fachgebiet Bioanalytik
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/453130481?language=en
• 关键词: Functional; structural; annotation; small; proteins

We will apply proteomics approaches pioneered in our labs to discover the functional and structural context of the newly identified small proteins of Methanosarcina mazei and Salmonella typhimurium of the initial phase of SPP2002. Using protein co-expression data and machine learning we will place small proteins in the context of cellular pathways. Using co-elution analysis and in cell crosslinking mass spectrometry, methods that reveal protein-protein interactions, we will obtain a level of detail that will allow us to model their complexes. This information on the cellular pathways and structural contexts of these small proteins will guide functional experiments done by our collaborators Profs. Schmitz-Streit (M. mazei) and Erhard (S. typhimurium). Our mass spectrometric data will be shared openly through ProteomeXchange and our interaction data through our own web-based resources https://www.proteomehd.net/ and https://xiview.org/ and the widely used https://string-db.org/ (>100,000 users/week).

我们将应用蛋白质组学的方法，在我们的实验室中开创，发现新发现的小蛋白的功能和结构的背景下的甲烷八叠球菌和鼠伤寒沙门氏菌的SPP 2002的初始阶段。利用蛋白质共表达数据和机器学习，我们将把小蛋白质放在细胞通路的背景下。使用共洗脱分析和细胞交联质谱法，揭示蛋白质-蛋白质相互作用的方法，我们将获得一定程度的细节，使我们能够模拟它们的复合物。这些关于这些小蛋白的细胞通路和结构背景的信息将指导我们的合作者教授所做的功能实验。Schmitz-Streit（M. mazei）和埃哈德（S.鼠伤寒沙门氏菌）。我们的质谱数据将通过ProteomeXchange公开共享，我们的相互作用数据将通过我们自己的基于网络的资源https://www.proteomehd.net/和https://xiview.org/以及广泛使用的https://string-db.org/（> 100，000用户/周）共享。