Investigating the role of individual dopaminergic reward neurons for locomotion

研究单个多巴胺能奖励神经元对运动的作用

• 批准号: 22K20673
• 负责人: Schleyer Michael
• 金额: $ 1.83万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-08-31 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K20673/
• 关键词: Locomotion; Dopamine; Drosophila; Parkinson; Parkinson Disease; Video-tracking

The most important achievement is the publication of our novel behavioural tracking software that we are using. The manuscript has been accepted now and will be published within the next days. This publication includes the original experiments on which this project is based on and new experiments that were not finished at the time of the proposal. This also means that our novel software that we will used throughout the project is fully ready and all further behavioural analyses can be completed speedily.We established the working setups and the basic experiments in our new lab in Sapporo. Also, we recruited an undergraduate and a Master student. All of these efforts took a bit longer than expected, but now we are ready for all planned experiments.The Master student already started experiments testing for the molecular basis of the observed locomotion phenotypes. Towards this end, we decided to first employ a drug treatment to inhibit the production of dopamine, before investigating individual dopamine receptors. The first results look very promising.Also, we started to check the first Parkinson's disease fly models for behavioural and anatomical phenotypes.

最重要的成就是我们正在使用的新型行为跟踪软件的出版。手稿已经被接受，将在未来几天内出版。该出版物包括该项目所基于的原始实验以及在提案时尚未完成的新实验。这也意味着我们将在整个项目中使用的新型软件已经完全准备就绪，所有进一步的行为分析都可以快速完成。我们在札幌的新实验室建立了工作设置和基本实验。此外，我们还招募了一名本科生和一名硕士生。所有这些工作都比预期的要长一些，但现在我们已经准备好了所有计划的实验。硕士生已经开始实验测试观察到的运动表型的分子基础。为此，我们决定在研究单个多巴胺受体之前，首先采用药物治疗来抑制多巴胺的产生。第一个结果看起来非常有希望。同时，我们开始检查第一个帕金森病苍蝇模型的行为和解剖表型。

项目成果

Page to describe our new tracking software

描述我们新的跟踪软件的页面

How dopamine neurons direct the search for food and safety in Drosophila larvae

多巴胺神经元如何指导果蝇幼虫寻找食物和安全

• 发表时间: 2022
• 作者: Michael Thane;Emmanouil Paisios;Torsten Stoeter;Anna-Rosa Krueger;Sebastian Glaess;Anne-Kristin Dahse;Nicole Scholz;Bertram Gerber;Dirk J. Lehmann;Michael Schleyer;三田真理恵;Michael Schleyer
• 通讯作者: Michael Schleyer