Functional and molecular profiling of cellular diversity and identity of the intermediate nucleus of the lateral lemniscus – a largely unexplored auditory center.

外侧丘系中间核的细胞多样性和身份的功能和分子分析 - 一个很大程度上未被探索的听觉中心。

• 批准号: 453200017
• 负责人: Professor Dr. Felix Felmy
• 金额: --
• 依托单位: Institut für Zoologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/453200017?language=en
• 关键词: Functional; molecular; profiling; cellular; diversity

The three auditory nuclei in the lateral lemniscus are thought to be important filters for central sound processing. The function and cellular features of the two inhibitory, the dorsal and the ventral nucleus, have been described und partially elucidated. However, the function and the cellular features of the glutamatergic neurons in the intermediate nucleus of the lateral lemniscus (INLL) are largely unexplored. Contrary to most other auditory nuclei, our preliminary data indicates a large range of integration time windows in INLL neurons and a cellular heterogeneity. To gain insights into the cellular features underlying sound processing in the INLL, we plan to investigate the cellular physiology of INLL neurons and compare these to the molecular cell heterogeneity. Combining whole-cell recordings and Patch-seq based single cell transcriptome analysis, we seek to identify candidate transcripts defining specific sub-populations and elucidate the integration properties of distinct INLL neurons. This proposal, therefore, generates the cellular understanding for illuminating the functional significance of the INLL. This proposal is submitted in the format of a ‘package proposal’, together with a proposal by Eckhard Friauf. In our final work package, we will combine the datasets on INLL neurons with Friaufs’s on the lateral superior olive for joint assessment within a meta-analysis.

外侧小网膜中的三个听核被认为是中央声音处理的重要过滤器。两个抑制的功能和细胞特征，背核和腹侧核，已被描述和部分阐明。然而，关于外侧小丘中间核谷氨酸能神经元的功能和细胞特征，目前还未得到充分的研究。与大多数其他听核相反，我们的初步数据表明INLL神经元的整合时间窗范围很大，并且细胞异质性。为了深入了解INLL中声音处理的细胞特征，我们计划研究INLL神经元的细胞生理学，并将其与分子细胞异质性进行比较。结合全细胞记录和基于Patch-seq的单细胞转录组分析，我们试图识别定义特定亚群的候选转录本，并阐明不同INLL神经元的整合特性。因此，这一建议产生了阐明INLL功能意义的细胞理解。该提案以“一揽子提案”的形式提交，与Eckhard Friauf的提案一起提交。在我们最后的工作包中，我们将把INLL神经元的数据集与Friaufs关于侧上橄榄的数据集结合起来，在荟萃分析中进行联合评估。