Development of immunomodulatory scaffolds for tissue regeneration

用于组织再生的免疫调节支架的开发

• 批准号: 22K20989
• 负责人: LARANJEIRA・ABE GABRIELA
• 金额: $ 1.83万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-08-31 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K20989/
• 关键词: Scaffolds; Macrophages; Immunomodulation; Biomaterials; Tissue regeneration

Our poly(lactic acid/caprolactone) copolymer (PLCL) technology was used to fabricate porous scaffolds of different sizes, from 200 um to 2.5 mm thickness. This achievement means that PLCL scaffolds can be adapted to numerous applications in tissue regeneration, and are not limited to bone regeneration as initially hypothesized in our proposal. Gas plasma treatment of PLCL scaffolds was performed, and new functional groups were detected by FTIR-spectroscopy. However, field emission SEM revealed different degrees of structural damage. Macrophages cultured on PLCL scaffolds showed a proliferation rate comparable to that of control materials; however, signs of scaffold degradation were observed by SEM, indicating that macrophages were engaged in the breakdown and phagocytosis of PLCL scaffold.

我们的聚乳酸/己内酯共聚物(PlcL)技术被用来制备不同尺寸的多孔支架，厚度从200微米到2.5毫米。这一成果意味着PLcL支架可以适应组织再生的多种应用，而不是像我们最初建议的那样局限于骨再生。对PLCL支架进行了气体等离子体处理，并用FTIR光谱检测到新的官能团。然而，场发射扫描电子显微镜显示出不同程度的结构损伤。在PlcL支架上培养的巨噬细胞的增殖率与对照材料相当，但扫描电子显微镜观察到支架降解的迹象，表明巨噬细胞参与了PlcL支架的破坏和吞噬。

项目成果

ORCID

• DOI: 10.5195/jmla.2017.89
• 发表时间: 2017-04
• 期刊: Journal of the Medical Library Association : JMLA
• 作者: Evan Sprague

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