In vitro study of the association between ALDH2 rs671 polymorphism and Acral Lentiginous Melanoma

ALDH2 rs671多态性与肢端雀斑样黑色素瘤关联的体外研究

• 批准号: 22K21117
• 负责人: Ashenagar M・Said
• 金额: $ 1.83万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-08-31 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22K21117/
• 关键词: Melanoma; Melanocytes; Melanin; Keratinocytes; Ethanol; Acetaldehyde; Alcohol; ALDH2; Cancer; ALM

Malignant melanoma is a highly aggressive cancer that primarily originates from melanocytes in the epidermis. Over the past few decades, its incidence has been steadily increasing. Acral Lentiginous Melanoma (ALM) is a subtype of melanoma that develops on the palms and soles and is associated with a particularly poor prognosis. This project aims to explore whether Acral Lentiginous Melanoma is linked to acetaldehyde, a potentially relevant factor. To address this question, we first conducted an evaluation of melanin production using different reagents, including Acetaldehyde (ranging from 10 to 10^4 μM), Ethanol (0.01%), and α-MSH (at concentrations of 10^3 and 10^4 μM). The evaluation was performed at 6 and 24-hour time points, comparing the results to a control group. Intracellular melanin content was quantified using colorimetric methods. The initial findings indicate that acetaldehyde and α-MSH, at specific concentrations and time points, have the potential to influence intracellular melanin production in melanoma cells. However, the impact of ethanol seems to be negligible based on the tested concentrations. Further analysis and additional experiments are necessary to fully comprehend the underlying mechanisms and validate these preliminary findings.

恶性黑色素瘤是一种高度侵袭性的癌症，主要起源于表皮中的黑素细胞。在过去的几十年里，其发病率一直在稳步上升。肢端长痣性黑色素瘤（ALM）是黑色素瘤的一种亚型，发生在手掌和脚底，预后特别差。该项目旨在探索肢端长痣性黑色素瘤是否与乙醛有关，这是一个潜在的相关因素。为了解决这个问题，我们首先使用不同的试剂进行了黑色素生成的评价，包括乙醛（范围为10 - 10^4 μM）、乙醇（0.01%）和α-MSH（浓度为10^3和10^4 μM）。在6和24小时时间点进行评价，将结果与对照组进行比较。使用比色法定量细胞内黑色素含量。初步研究结果表明，乙醛和α-MSH在特定浓度和时间点有可能影响黑色素瘤细胞中细胞内黑色素的产生。然而，根据检测浓度，乙醇的影响似乎可以忽略不计。进一步的分析和额外的实验是必要的，以充分理解潜在的机制，并验证这些初步发现。