New practical technique for Protein Crystallization with Mebiol Gel

在这项研究中，我们成功地在Mebiol凝胶结晶三种蛋白质。我们的分析表明，Mebiol凝胶为克服蛋白质高温损伤的固有问题提供了新的可能性。我们还研究了Mebiol凝胶存在下蛋白质晶体的成核速率，发现Mebiol凝胶增加了蛋白质晶体的数量。此外，围绕晶体的Mebiol凝胶减少了高离子强度溶液中渗透压休克的影响。该技术将为生物大分子在水凝胶中的结晶开辟新的维度。

In this study, we successfully crystallized three proteins in Mebiol gel. Our analysis indicates that Mebiol gel provides new possibilities for overcoming the inherent problem of high-temperature damage to the proteins. We also investigated the nucleation rate of protein crystals in the presence of Mebiol gel and found that Mebiol gel increased the number of protein crystals. Furthermore, the Mebiol gel surrounding the crystals reduces the influence of osmotic shock in high-ionic-strength solutions. This technique will open up a new dimension for the crystallization of biological macromolecules in hydrogels.