Construction of in-body self-organized tissue fabrication bioprocess for the realization of novel regenerative medicine

BIOTUBEs是利用体内组织结构技术（IBTA）制备的血管移植物，具有良好的力学性能和良好的生物相容性，是再生医学中最有前途的血管移植物之一。然而，它们的壁非常薄，仅由结缔组织组成，主要是胶原纤维和成纤维细胞以及内皮细胞，这可能导致血栓闭塞。在本研究中，通过IBTA与脂肪源性血管基质细胞（ADSCs）的结合，成功制备了具有内皮细胞和平滑肌细胞成分的血管样组织。此外，通过巨大滴滴的ADSCs球体修补，也实现了BIOTUBE移植物内皮化的快速成熟。这些技术被认为是实现心血管组织乃至其他组织再生医学的有前途的关键技术。

BIOTUBEs, prepared by using in-body tissue architecture technology (IBTA), are one of the most promising vascular grafts in regenerative medicine because of their adequate mechanical property and excellent biocompatibility as vascular grafts. However, their walls were very thin and composed of only connective tissues with mainly collagen fiber and fibroblasts and luck of endothelium, which may lead to thrombotic occlusion. In this study, vascular-like tissues with endothelial cells and smooth muscle cells components were successfully prepared by the combination of IBTA and delivery of adipose-derived vascular stromal cells (ADSCs) from the specially designed porous mold. Additionally, rapid maturation with endothelialization of BIOTUBE grafts was also achieved by patching of giant drops of ADSCs spheroids. These technologies are considered to be a promising key-technology for the realization of not only cardiovascular tissues but also other tissues regenerative medicine.