Coherence of directional sensing, polarity, and motility in collective cell migration

集体细胞迁移中方向感知、极性和运动的一致性

• 批准号: 23870006
• 负责人: NAKAJIMA Akihiko
• 金额: $ 2.08万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23870006/
• 关键词: 細胞運動; 粘菌; 走化性; 適応; 自己組織化; 反応拡散波; 振動; 集団的細胞移動; cAMP振動

Chemotaxis is one of basic cellular functions to response to environment. We have studied multicellular effect on chemotaxis in slime mold Dictyostelium discoideum. Migration behaviors during aggregation were analyzed quantitatively. It was found that cells harboring different Tgr proteins separate within a few minutes after contact, while cells harboring the same proteins migrated together. Cells surrounded by the same type moved faster and aligned polarities with others. Such coordinated behaviors were observed in only two cells isolated from aggregate. Taken together, self/non-self recognition via Tgr proteins modulates collective migration behavior. We also developed a microfluidics system to generate spatio-temporal chemoattractant field artificially. Using this system, we demonstrated cells were able to migrate directionally in such chemoattractant field.

趋化性是细胞对环境反应的基本功能之一。本文研究了盘基网柄霉（Dictyosterichdiscoideum）趋化性的多细胞效应。对聚集过程中的迁移行为进行了定量分析。发现携带不同Tgr蛋白的细胞在接触后几分钟内分离，而携带相同蛋白的细胞一起迁移。被相同类型包围的细胞移动得更快，并与其他细胞对齐极性。这种协调的行为，观察到只有两个细胞从聚集体分离。总之，通过Tgr蛋白的自我/非自我识别调节集体迁移行为。我们还开发了一个微流控系统，人工产生时空化学引诱场。使用该系统，我们证明细胞能够在这样的化学引诱物场中定向迁移。

项目成果

細胞性粘菌（研究の新展開）～モデル生物、創薬資源、バイオ～

细胞粘菌（研究新进展）-模式生物、药物发现资源、生物技术-

• 发表时间: 2012
• 作者: 阿部知顕;前田靖男（編著）;久保原禅 他（著）
• 通讯作者: 久保原禅 他（著）

The green tea catechin epigallocatechin gallate (EGCG) blocks cell motility, chemotaxis and development in Dictyostelium discoideum.

• DOI: 10.1371/journal.pone.0059275
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: McQuade KJ;Nakajima A;Ilacqua AN;Shimada N;Sawai S
• 通讯作者: Sawai S

Temporal organization of chemoattractant field and collective cell migration

趋化剂场的时间组织和集体细胞迁移

• 发表时间: 2011
• 作者: Akihiko Nakajima;Shuji Ishihara;Sawai Satoshi;岩井良輔;永井大樹，大山聖，得竹浩，竹内伸介，安養寺正之;中島昭彦
• 通讯作者: 中島昭彦

Directed cell migration induced by travelling waves of chemoattractant

趋化剂行波诱导细胞定向迁移

• 发表时间: 2012
• 作者: Akihiko Nakajima;Shuji Ishihara;Sawai Satoshi
• 通讯作者: Sawai Satoshi

自己組織化する集合シグナルと協調的な細胞運動

自组织集体信号和协调细胞运动

• 发表时间: 2011
• 作者: Ozawa;M.;Kawakami;E.;Sakamoto;R.;Shibasaki;T.;Goto;A. and Yoshida;N.;中島昭彦
• 通讯作者: 中島昭彦