The green tea catechin epigallocatechin gallate (EGCG) blocks cell motility, chemotaxis and development in Dictyostelium discoideum.
The green tea catechin epigallocatechin gallate (EGCG) blocks cell motility, chemotaxis and development in Dictyostelium discoideum.
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DOI：
10.1371/journal.pone.0059275
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Sawai S
中科院分区：
文献类型：
--
作者：
McQuade KJ;Nakajima A;Ilacqua AN;Shimada N;Sawai S
儿茶素是在绿色茶中发现的高含量黄烷醇，由于其与癌症、自身免疫和代谢疾病相关的潜在健康益处而受到广泛关注，但人们对这些化合物影响细胞行为的机制知之甚少。在这里，我们评估是否模式生物Dictyosteelium discoideum是一个有用的工具，其特征儿茶素的影响。表没食子儿茶素没食子酸酯（Epigallocatechin gallate，EGCG）是绿色茶中含量最丰富、作用最强的儿茶素，对网囊藻的生命周期有重要影响。在存在EGCG的情况下，聚集被延迟，细胞不流动，并且发育通常停滞在松散聚集阶段。发育的影响很可能是由于运动缺陷，因为EGCG减少了Dictyosteomelamoeomelae的随机运动和趋化性。这些结果表明，儿茶素及其衍生物可能是有用的工具，以更好地了解细胞的运动性和发展，在Dictyosteoblastoma和这种生物体是一个有用的模型，以进一步表征儿茶素的活动。
Catechins, flavanols found at high levels in green tea, have received significant attention due to their potential health benefits related to cancer, autoimmunity and metabolic disease, but little is known about the mechanisms by which these compounds affect cellular behavior. Here, we assess whether the model organism Dictyostelium discoideum is a useful tool with which to characterize the effects of catechins. Epigallocatechin gallate (EGCG), the most abundant and potent catechin in green tea, has significant effects on the Dictyostelium life cycle. In the presence of EGCG aggregation is delayed, cells do not stream and development is typically stalled at the loose aggregate stage. The developmental effects very likely result from defects in motility, as EGCG reduces both random movement and chemotaxis of Dictyostelium amoebae. These results suggest that catechins and their derivatives may be useful tools with which to better understand cell motility and development in Dictyostelium and that this organism is a useful model to further characterize the activities of catechins.
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影响因子：
5.6
作者：
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通讯作者：
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影响因子：
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作者：
Kotsifas, M;Barth, C;Fisher, PR
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影响因子：
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作者：
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通讯作者：
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DOI：
10.1007/s10856-010-4019-3
发表时间：
2010-05-01
影响因子：
3.7
作者：
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通讯作者：
Burt, Helen M.