Developmental study of the naso-hypophyseal placode in cyclostomes (Lamprey and Hagfish)

在这项研究中，我们发现，虽然七鳃鳗鼻-垂体胎盘发育为单一的中间增厚，但在胎盘增厚之前，鼻部和垂体部的胎盘特异基因已经分别表达。为了追踪鼻侧胎盘区域的细胞谱系，我们成功地在胎盘前区域局部注射了DII。此外，为了追踪细胞的运动，我们在1个细胞期注射了荧光蛋白，并成功地观察到了这只动物的荧光素信号。关于鳗鱼，我们获得了一些受精卵。我们正准备分析它们的基因表达模式。

In this study, we found that although lamprey naso-hypophyseal placode develops as a single median thickening, nasal- and hypophyseal placode specific genes were already expressed separately before placode thickening. In order to track the cell lineage of the nasal placodal area, we succeeded to inject DiI focally at the pre-placodal area. Also, to trace cell movements, we injected a fluorescence protein at 1 cell stage and succeeded to observe fluorescein signals in this animal. Regarding hagfishes, we obtained a few fertilized eggs. We are preparing to analyze their gene expression patterns.