Developmental study of the naso-hypophyseal placode in cyclostomes (Lamprey and Hagfish)

在本研究中，我们发现尽管七鳃鳗鼻-垂体基板以单一中间增厚的方式发育，但在基板增厚之前，鼻腔和垂体基板特异性基因已经分别表达。为了追踪鼻腭区细胞谱系，我们成功地在鼻腭前区局部注射了DiI。此外，为了追踪细胞运动，我们在该动物的一个细胞阶段注射了荧光蛋白，并成功地观察到荧光素信号。关于盲鳗，我们获得了一些受精卵。我们正准备分析他们的基因表达模式。

In this study, we found that although lamprey naso-hypophyseal placode develops as a single median thickening, nasal- and hypophyseal placode specific genes were already expressed separately before placode thickening. In order to track the cell lineage of the nasal placodal area, we succeeded to inject DiI focally at the pre-placodal area. Also, to trace cell movements, we injected a fluorescence protein at 1 cell stage and succeeded to observe fluorescein signals in this animal. Regarding hagfishes, we obtained a few fertilized eggs. We are preparing to analyze their gene expression patterns.