Construction of one-batch system for glycansynthesisusing enzymes showing different optimal temperature

使用具有不同最适温度的酶构建一次性聚糖合成系统

• 批准号: 23880016
• 负责人: TSURUTA Sachiko
• 金额: $ 1.91万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2011
• 资助国家: 日本
• 起止时间: 2011 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-23880016/
• 关键词: 好冷菌; 低温活性酵素; 糖転移酵素; 糖鎖

The aim of this study is construction of one-batch system for glycan synthesis using enzymes showing different optimal temperature. To achieve the goal, we first cloned 6 genes of glycosyltransferase, group 2 family proteins (GT-2) from Shewanella frigidimarina, and constructed expression system of GT-2 proteins. As a result, we obtained two soluble recombinant GT-2s, using the expression vector derived from pCold I. Surveying the protein structure by X-ray analysis will enables us to clarify “structural factors” for eliciting cold-activity. Based on the results, we will create new types of GT-2 enzymes with cold-activity.

这项研究的目的是使用显示出不同最佳温度的酶建造用于聚糖合成的一批次系统。为了实现目标，我们首先克隆了6个基因的糖基转移酶，Shewanella Frigidimarina的2组家族蛋白（GT-2），以及GT-2蛋白的构建表达系统。结果，我们使用源自X射线分析的PCOLD I的表达向量获得了两个固体重组GT-2S，这将使我们能够阐明“结构因子”以引起冷活动。根据结果​​，我们将创建具有冷活动的新型GT-2酶。