Development of the reverse genetics system for Rice stripe virus

点击翻译按钮获取中文摘要

Rice stripe virus (RSV) is a negative-strand RNA virus that infects Rice plants transmitted by small brown plant hopper. Due to the unavailability of reverse genetics systems, knowledge about how plant negative-strand RNA viruses multiply is limited. In this project, I tried to develop a reverse genetics system of RSV. To reconstitute an infectious ribonucleoprotein (RNP) complex, I succeeded to express and purify the nucleocapsid protein and RNA polymerase of RSV, components of RSV RNP. Although mixtures of these proteins with viral RNA did not result in formation of the infectious RNP, the materials obtained in this project would help further development of the study of plant negative-strand RNA viruses.