The development of therapy for inflammatory bal tissue with Paneth cell-protection factorsowel disease using cultured epitheli

本研究的目的是分析肠道隐窝结构再生的机制，建立新的培养体系，以维持正常的隐窝结构。本研究从小鼠小肠中剥离肠绒毛和隐窝，并收集富隐窝部分进行培养。将小肠隐窝37度混合在马蒂凝胶中，覆盖干细胞选择培养液。小肠隐窝在培养过程中不进行细胞分离。检查了培养基中的几个因素，发现R-响应蛋白是影响培养体系的关键因素。为了建立动物模型，对狗和猪的组织进行了病理学分析。在猪肠中可见富含颗粒的Paneth细胞，而在狗中可见。猪是替代人类研究的动物模型候选之一。动物模型的发展被寄予厚望。

The aim of this research is to analyze the mechanisms in regeneration of crypt structures in the gut, and to establish novel culture system with maintaining the normal crypt structure.In this study, intestinal villi and crypt are exfoliated from mouse small intestine and the crypt-rich fractions are collected for the culture. Small intestinal crypts are mixed in Marti-gel at 37 degree, and covered with stem cell selection media. The small intestinal crypts are cultured without cell isolation. Several factors in culture media are checked and R-spondin is found to be a critical factor for culture system.To establish the animal model, the tissues of dog and pig are analyzed pathologically. Paneth cells with rich granules are observed in the porcine intestine but in the dog. Pig is one of the candidates for animal model alternative to human research. The development of animal model is highly expected in future.