Analytical 4-Laser flow cytometer

分析型 4 激光流式细胞仪

• 批准号: 453716058
• 金额: --
• 依托单位: Universität Hamburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2020
• 资助国家: 德国
• 起止时间: 2019-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/453716058?language=en
• 关键词: Analytical; Laser; flow; cytometer

Beside two cell sorters and an Imaging cytometer, the Cytometry & Cell Sorting (CCS) Core Unit of the University Medical Centre Hamburg-Eppendorf (UKE) has got three analytical flow cytometers, particularly a 5-laser one (20 colours), a 4-laser one (17 colours) and a 3-laser device (8 colours). All analytical flow cytometers have had a high to very high workload. With the installation of the 5-laser device the CCS core can now offer multi-parameter-analyses with up to 20 colours thus covering the increasing need in this area. However, many of the standard applications in the core have so far comprised 3 to 8 colours. For these measurements, most users have been happy with the 3-laser flow cytometer in the last years, which have been quite popular (app. 1200 h pure analysis time [excluding maintenance, service etc.] in 2019). Unfortunately, the 3-laser device was obtained back in 2007 and is now 13 years old, which has resulted in increasing numbers of and costs for repairs. Consequently, we urgently need a replacement, which builds on the parameters of the current device, but also on the recent progress in flow cytometry. The latter particularly includes instalment of a fourth, yellow-green laser (and the respective detectors) in order to enable detection of red proteins now frequently used by many of the core users as well as novel red fluorophores. Moreover, the new flow cytometer should allow measurement of sample volumes and thus absolute cell counts, which will, for example, facilitate high-throughput toxicity analyses, since direct sampling will be possible from 96-well as well as 384-well plates.Altogether, the current 13-year old device in the Core Facility Cytometry & Cell Sorting needs to be replaced by a novel medium-class flow cytometer. This will warrant the continuous work of the Cytometry & Cell Sorting Core Unit of the UKE and, moreover, establish novel opportunities for the user in accord with the current state-of-the-art in analytical flow cytometry.

除了两台细胞分选仪和一台成像细胞仪外，汉堡-埃彭多夫大学医学中心 (UKE) 的细胞计数和细胞分选 (CCS) 核心单位还拥有三台分析流式细胞仪，特别是一台 5 激光流式细胞仪（20 种颜色）、一台 4 激光流式细胞仪（17 种颜色）和一台 3 激光设备（8 种颜色）。所有分析流式细胞仪都具有高至非常高的工作量。随着 5 激光设备的安装，CCS 核心现在可以提供多达 20 种颜色的多参数分析，从而满足该领域日益增长的需求。然而，迄今为止，核心中的许多标准应用都包含 3 到 8 种颜色。对于这些测量，大多数用户对过去几年非常受欢迎的 3 激光流式细胞仪感到满意（2019 年纯分析时间约为 1200 小时[不包括维护、服务等]）。不幸的是，该 3 激光设备是 2007 年获得的，现在已有 13 年的历史，这导致维修数量和成本不断增加。因此，我们迫切需要一种替代品，它不仅基于当前设备的参数，而且还基于流式细胞术的最新进展。后者特别包括安装第四个黄绿色激光器（以及相应的检测器），以便能够检测现在许多核心用户经常使用的红色蛋白质以及新型红色荧光团。此外，新的流式细胞仪应允许测量样品体积，从而测量绝对细胞计数，这将有助于高通量毒性分析，因为可以从 96 孔板和 384 孔板直接采样。总之，核心设施细胞计数和细胞分选中当前已有 13 年历史的设备需要更换为新型中级流式细胞仪。这将保证 UKE 的细胞计数和细胞分选核心单元的持续工作，此外，根据当前分析流式细胞术的最新技术，为用户创造新的机会。