Analytical 5-laser multi-parameter flow cytometer

在单细胞水平上对异质样本中的细胞进行多参数分析是在健康和疾病背景下理解生物关系和机制的最重要的技术之一。关键技术是使用流式细胞术或质量细胞术分析悬浮中的单个细胞，以及使用组织病理学或成像质量细胞术分析组织环境中的细胞。流式细胞术使细胞能够在高通量下使用各种参数进行分析。对用于细胞分析和细胞分选的高度专业化设备的高需求以及高昂的成本要求集中提供这种技术。通过集成到中央核心设施中，可以有效地使用仪器并为用户提供专家支持。10多年来，中央流动和质量细胞仪核心设施‘Charité|BIH细胞仪核心’一直在CharitéCVK现场为多参数单细胞分析和细胞分选提供专家服务和最新设备。然而，CharitéMitte站点目前没有集中访问流式细胞仪的设备。CharitéMitte站点的工作组参与这项申请，依赖于在这里为其跨学科研究项目使用所需的设备。到目前为止，来自其他研究所的设备可以用于一些项目，或者分析可以分成几种方法，参数更少。其他研究所的设备在必要的程度上不再可用。样品材料的局限性对同时分析尽可能多的参数提出了越来越高的要求。因此，Charité的S中心流式细胞仪核心设备申请购买分析型5激光多参数流式细胞仪。与5激光高速细胞分选设备一起，该仪器应构成中央核心设施扩展的基础，也超出了这里列出的申请者群体。在一个共同的管理结构下，将该仪器纳入当时跨地点的中央核心设施，应可确保技术迅速到位。已建立的质量管理结构被接管，并通过经过验证的培训概念保证对用户的培训。

The multi-parameter analysis of cells within a heterogeneous sample at the single cell level is one of the most important technology for understanding of biological relationships and mechanisms in the context of health and disease. Key technologies are the analysis of single cells in suspension using flow cytometry or mass cytometry, as well as the analysis of cells in the tissue context using histopathology or imaging mass cytometry. Flow cytometry enables cells to be analyzed using a variety of parameters at high throughput. The high demand for highly specialized devices for both cell analysis and cell sorting and the high costs argue for a centralized provision of such technologies. The integration into a central core facility allows efficient use of the instruments and expert support for the users.The central Flow & Mass Cytometry Core Facility 'Charité | BIH Cytometry Core ' has been offering expert service and the latest equipment for multi-parameter single cell analysis and cell sorting at the Charité CVK site for more than 10 years. However, there is currently no centralized access to flow cytometry devices at the Charité Mitte site.The working groups at the Charité Mitte location involved in this application are dependent on the use of the equipment requested here for their interdisciplinary research projects. So far, devices from other institutes could be used for some of the projects or analyzes could be broken down into several approaches with fewer parameters. The devices of the other institutes are no longer available to the extent necessary. The limitation of the sample material increasingly requires a simultaneous analysis of as many parameters as possible.The Charité's central Flow & Mass Cytometry Core Facility therefore applies here for the purchase of an analytical 5-laser multi-parameter flow cytometer. Together with a 5-laser high-speed cell sorting device, this instrument should form the basis for the expansion of the central core facility also beyond the group of the applicants listed here. The integration of the instrument into the then cross-location central core facility under a common management structure should ensure the rapid availability of the technology. Established quality management structures are taken over and the training of the users is guaranteed through proven training concepts.