LSRII Four Laser Analytical Flow Cytometer

LSRII 四激光分析流式细胞仪

• 批准号: 7047390
• 负责人: David A. Thorley-Lawson
• 金额: $ 30.72万
• 依托单位: TUFTS UNIVERSITY BOSTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047390
• 关键词: community; flow cytometry; lasers; measurement

DESCRIPTION (provided by applicant): We are applying for funds to support the purchase of instrumentation, necessary to serve the flow cytometry needs of the research community at Tufts University School of Medicine (TUSM) and Tufts-New England Medical Center (T-NEMC). The facility has been in existence for 17 years and has been run for the last 6 years by Mr. Allen Parmelee, a skilled and respected operator, under the direct supervision of the P.I. and the Core Advisory Committee. Under their direction the facility has grown, become highly successful and maintained financial stability through competitive pricing and Departmental support. 85% of facility time is dedicated to the NIH funded work of the Core User Group. We are now asking for an LSR II (BD Biosciences) analytical machine. This instrument will double our analytical capacity, while providing access to a powerful range of techniques not currently available to us, including the measurement of intracellular calcium in cells expressing GFP variants, using Indo-1 (355nm laser); the tracking of cell cycle, viability, and proliferation, using Hoechst or DAPI (355nm laser); the analysis of protein expression, using CFP (405nm laser); the measurement of molecular association at the nanometer scale, using CFP-to-YFP FRET (405nm laser) and any analysis requiring 5 or more colors. The cost efficiency and high standards of our facility have encouraged use to the point where the facility (one FACSCalibur and one, soon to be two, MoFLos) is now over-extended. In addition, new faculty hiring, combined with growing interest in flow applications throughout TUSM and, recently, T-NEMC has increased the size of our NIH funded Core User Group from 6 to 17. This renders the acquisition of a new LSR II essential, if we are to meet the flow cytometry needs, both in time and available technology, for the community of NIH funded researchers at TUSM and T-NEMC.

描述(由申请人提供)：我们正在申请资金，以支持购买仪器，以满足塔夫茨大学医学院(TUSM)和塔夫茨-新英格兰医学中心(T-NEMC)研究社区的流式细胞术需求。该设施已经存在了17年，过去6年一直由Allen Parmelee先生运营，他是一位经验丰富和受人尊敬的操作员，在私人情报局和核心咨询委员会的直接监督下。在他们的指导下，该设施已经发展壮大，变得非常成功，并通过具有竞争力的定价和部门支持保持了财务稳定。85%的设施时间专门用于NIH资助的核心用户组的工作。我们现在需要一台LSR II(BD生物科学)分析机。这台仪器将使我们的分析能力翻一番，同时提供我们目前无法利用的一系列强大技术，包括使用INDO-1(355 nm激光)测量表达GFP变体的细胞内钙离子；使用Hoechst或DAPI(355 nm激光)跟踪细胞周期、活力和增殖；使用CFP(405 nm激光)分析蛋白质表达；使用CFP-to-YFP FRET(405 nm激光)在纳米尺度测量分子缔合以及需要5种或更多颜色的任何分析。我们设施的成本效益和高标准鼓励使用，以至于设施(一个FACSCalibur和一个即将成为两个，MoFlos)现在已经过度扩张。此外，新教师的招聘，以及整个TUSM和T-NEMC对Flow应用程序日益增长的兴趣，使我们由NIH资助的核心用户组的规模从6个增加到17个。这使得如果我们要满足NIH资助的TUSM和T-NEMC研究人员社区在时间和可用的技术方面的需求，购买新的LSR II是必不可少的。