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Analyses of molecular mechanism of yellow symptoms induced by Cucumber mosaic virus Y satellite RNA

黄瓜花叶病毒Y卫星RNA引起黄色症状的分子机制分析

基本信息

批准号：
24880005
负责人：
SHIMURA Hanako
金额：
$ 1.91万
依托单位：
Hokkaido University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Research Activity Start-up
财政年份：
2012
资助国家：
日本
起止时间：
2012-08-31 至 2014-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24880005/
关键词：
ウイルスサテライトRNA RNAサイレンシング病徴誘導

项目摘要

Cucumber mosaic virus (CMV) Y satellite RNA (Y-sat) specifically induces a bright yellow mosaic on tobacco through RNA silencing machinery. In this study, I investigated the roles of major RNA silencing components such as DCLs and RDR6 in the Y-sat-mediated yellow induction. By using a virus-induced gene silencing strategy against DCLs and RDR, I found that the accumulation levels of CMV RNAs, Y-sat, DCL2, DCL4 and RDR6 mRNAs changed in a complicated manner interfering with each other. Based on transcript analyses, it was found that DCL4 was more important than DCL2 in affecting the accumulation levels of CMV and Y-sat and thus to generate 21-nt siRNAs of Y-sat. It was also found that Y-sat siRNA levels were positively correlated with the degree of yellowing. In addition, the yellow induction by Y-sat was apparently independent of RDR6, but RDR6 indirectly affects the yellow symptoms because decreased levels of RDR6 enhanced Y-sat siRNA levels and eventually enhanced yellowing.

黄瓜花叶病毒（CMV）Y卫星RNA（Y-sat）通过RNA沉默机制特异性地诱导烟草上的亮黄色花叶。在这项研究中，我研究了主要的RNA沉默组件，如DCLs和RDR 6在Y-sat介导的黄色诱导中的作用。通过使用针对DCL和RDR的病毒诱导的基因沉默策略，我发现CMV RNA、Y-sat、DCL 2、DCL 4和RDR 6 mRNA的积累水平以相互干扰的复杂方式变化。基于转录物分析，发现DCL 4在影响CMV和Y-sat的积累水平方面比DCL 2更重要，从而产生Y-sat的21-nt siRNA。还发现Y-sat siRNA水平与黄化程度正相关。此外，Y-sat的黄化诱导明显不依赖于RDR 6，但RDR 6间接影响黄化症状，因为RDR 6水平的降低增强了Y-sat siRNA水平并最终增强了黄化。