Identification of genetic factors affect the success or failure of the transformation in barley

我们利用大麦cv产生的群体图谱探索了遗传转化所需的基因组区域。黄金希望，是一个可靠的转化品种。因此，1H、2H、3H、6H和7H染色体的部分区域被估计为候选基因的位置。然后，通过基因表达谱芯片的综合分析，筛选出了4308个在金色承诺中表达模式特异的基因。在植物激素的分析中，我们发现生长素对大麦愈伤组织的再生有积极的作用，而脱落酸和水杨酸对愈伤组织的再生有负面影响。

We explored the genomic regions required for genetic transformation by using a mapping populations generated from barley cv. Golden Promise, which was a reliable cultivar for transformation. As a result of this, the partial region of chromosome No. 1H, No. 2 H, No. 3H, No. 6 H and No. 7 H were estimated as the positions of candidate genes. Then we isolated 4308 genes which expression patterns were specific in Golden Promise by a comprehensive gene expression analysis using microarray. In the analysis of phytohormone, we identified that auxin showed a positive effect in regeneration of barley callus, although abscisic acid and salicylic acid showed negative.