Establishment of method of dental pulp stem cell differentiation to Neural cell.

牙髓干细胞分化为神经细胞方法的建立。

• 批准号: 24890024
• 负责人: SUGAWARA Yu
• 金额: $ 1.91万
• 依托单位: Tohoku University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-08-31 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24890024/
• 关键词: 歯髄幹細胞; オリゴデンドロサイト; PDGF; 再生; 歯

PDGF-AA promotes dental pulp stem cell differentiation into odontoblast, whereas PDGF-BB promotes proliferation and differentiation of dental pulp stem cell into oligodendrocyte via PDGFRb-ERK1/2 and Akt pathway. Further, PDGF-BB inhibits BMP2 induced dental pulp stem cells differentiation into odontoblast inhibiting Smad5 phosphorylation.From these results, PDGF-BB may regulate oligodendrocyte differentiation from dental pulp stem cells, but not odontoblasts because of the differentiation marker expression after PDGF-BB stimulation. It is important information about the determination of cell fate of dental pulp stem cells and regeneration of neural using dental cells.

PDGF-AA促进牙髓干细胞向成牙本质细胞分化，而PDGF-BB通过PDGFRb-ERK 1/2和Akt途径促进牙髓干细胞增殖并向少突胶质细胞分化。此外，PDGF-BB抑制BMP 2诱导的牙髓干细胞向成牙本质细胞的分化，抑制Smad 5磷酸化。从这些结果来看，PDGF-BB可能调节牙髓干细胞向少突胶质细胞的分化，而不是向成牙本质细胞的分化，因为PDGF-BB刺激后分化标记物的表达。这对牙髓干细胞的细胞命运决定和利用牙源性细胞进行神经再生具有重要意义。

项目成果

Dental pulp stem cell differentiation regulated by PDGFs.

PDGFs 调节牙髓干细胞分化。

• 发表时间: 2013
• 作者: Yu Sugawara;Yu Sugawara
• 通讯作者: Yu Sugawara