Investigations for lincRNA functions involved in cardiac remodeling.

在本研究中，我们重点研究了lincRNAs，这是一种可能参与心肌肥大的非编码RNA。我们对小鼠腹主动脉缩窄后心脏表达上调的lincRNAs进行筛选，鉴定出3个lincRNAs。在本研究中，我们试图阐明lincRNAs的功能。首先，我们在新生小鼠心肌细胞中过表达了lincRNAs，发现心肌细胞肥大。此外，我们还证明，当两个lincRNAs过表达时，心肌肥厚的标志物ANP和BNP显著增加。此外，我们试图评估lincRNA是否与microRNA结合。我们发现lincRNA可以与一些对肥大起抑制作用的microRNAs结合。最后，我们发现，在过表达lincRNA的新生心肌细胞中，细胞周期蛋白的水平显著增加。这些结果表明，lincRNAs可能参与了心肌肥厚。

In the present study, we focused on the lincRNAs, a non-coding RNA, which may be involved in cardiac hypertrophy. We screened lincRNAs which were upregulated in mouse heart subjected to transverse aortic constriction, and identified three lincRNAs. In the present study, we tried to clarify the functions of the lincRNAs. Firstly, we overexpressed the lincRNAs in neonatal mouse cardiomyocytes, and found that cardiomyocyte hypertrophy was induced. Also, we demonstrated that ANP and BNP, markers of cardiac hypertrophy, were significantly increased when the two lincRNAs were overexpressed. Furthermore, we sought to evaluate whether a lincRNA bound to microRNAs. We found that lincRNA could bind to some microRNAs that play inhibitory role to hypertrophy. Finally, we revealed that protein level of cyclin was significantly increased in neonatal cardiomyocytes overexpressed the lincRNA. These results indicate that lincRNAs may be involved in cardiac hypertrophy.