Analysis of the role of genes involved in biofilm formation of Porphyromonas gingivalis

生物膜的细胞外基质由蛋白质、碳水化合物和核酸组成，具有保护微生物免受抗菌剂和免疫细胞侵害、稳定生物膜结构等功能。因此，传统的抗菌药物对生物膜感染的抑制作用较弱，开发一种针对细胞外基质而不是微生物细胞的新策略是控制生物膜的必要条件。本研究以牙周病的主要病原菌之一牙龈卟啉单胞菌（Porphyromonas gingivalis）的转录因子PGN_0088为研究对象，研究了PGN_0088对生物膜细胞外基质的作用。结果表明，PGN_0088对牙龈假单胞菌生物膜胞外基质碳水化合物合成有影响。

The extracellular matrix of the biofilm comprised of protein, carbohydrate and nucleic acid has been shown to functions such as protector of the microbe against antimicrobial agents and immune cells, and stabilizer of the biofilm structure. Therefore, traditional antimicrobial agents have less effect on suppression of biofilm infection, and the development of a novel strategy that targets extracellular matrix instead of microbial cells is necessary for control of the biofilm.In this study, I focused on an transcription factor PGN_0088 of Porphyromonas gingivalis, that is one of the main pathogen of periodontal disease, and examined how PGN_0088 works on the extracellular matrix in biofilm.As a result, it became clear that PGN_0088 effects on carbohydrate synthesis in the extracellular matrix of P. gingivalis biofilm.