Protective potentials of mannitol-erythropoietin against neurotoxicityof general anesthesia on immature brain.

研究表明，全身麻醉剂可在发育中的大脑中诱导广泛的细胞凋亡。我们研究了甘露醇-促红细胞生成素（M-Epo）联合给药是否对新生大鼠麻醉诱导的神经毒性有保护作用。我们发现全身麻醉6小时后，Caspase-3在大脑皮层和海马中被诱导，细胞凋亡增加。腹腔注射甘露醇和促红细胞生成素未能抑制细胞凋亡。麻醉大鼠血氧饱和度没有降低，但血二氧化碳浓度升高。我们的研究结果并不能说明M-Epo在抑制未成熟大脑麻醉诱导的神经毒性方面是否有效。

General anesthetics are shown to induce wide spread apoptosis in the developing brain. We studied if mannitol - erythropoietin (M-Epo) combined administration affords protection against anesthetic-induced neurotoxicity in neonatal rats. We found that Caspase-3 was induced in the cerebral cortex and hippocampus after 6 hour exposure to general anesthetics, meaning apoptosis increasing. Intraperitoneal administration of mannitol and Epo failed to inhibit apoptosis. In anesthetized rats, blood saturation of oxygen did not decreased, but blood concentration of carbon dioxide increased. Our results cannot suggest whether M-Epo is effective or not in inhibiting anesthetic-induced neurotoxicity in immature brain.