Protective potentials of mannitol-erythropoietin against neurotoxicityof general anesthesia on immature brain.

全身麻醉剂显示在发育中的大脑中诱导广泛的细胞凋亡。我们研究了甘露醇-促红细胞生成素（M-Epo）联合给药是否对新生大鼠麻醉诱导的神经毒性提供保护。结果发现，全麻药物作用6 h后，大脑皮层和海马中Caspase-3表达上调，细胞凋亡增加。腹腔注射甘露醇和Epo不能抑制细胞凋亡。在麻醉大鼠中，血氧饱和度没有降低，但血液中二氧化碳浓度增加。我们的结果不能表明M-Epo是否有效抑制麻醉诱导的未成熟脑神经毒性。

General anesthetics are shown to induce wide spread apoptosis in the developing brain. We studied if mannitol - erythropoietin (M-Epo) combined administration affords protection against anesthetic-induced neurotoxicity in neonatal rats. We found that Caspase-3 was induced in the cerebral cortex and hippocampus after 6 hour exposure to general anesthetics, meaning apoptosis increasing. Intraperitoneal administration of mannitol and Epo failed to inhibit apoptosis. In anesthetized rats, blood saturation of oxygen did not decreased, but blood concentration of carbon dioxide increased. Our results cannot suggest whether M-Epo is effective or not in inhibiting anesthetic-induced neurotoxicity in immature brain.