Genomic factors of parasites and human beings on malaria severity

红细胞上的CRI显示了一种遗传的数字多态性，其与泰国急性恶性疟疾患者CRI基因的HindIII-RFLP相关，因为他们的这种多态性的基因型。（ELISA）。我们观察到严重组中CRI低密度等位基因（LL）的纯合子频率显著高于无并发症组（P = 0.005）。LL基因型患者红细胞CRI表达显著低于高密度等位基因纯合子（HH）（P < 0.0001）和杂合子（HL）（P = 0.013）。结果表明，红细胞上遗传决定的低CRI密度可能是在该受试者中发展更严重形式的疟疾的风险因素。在Vanuatsu研究了H频率与疟疾流行的关系。实验性重症疟疾（ESM）是由伯氏疟原虫（PlasmodiumbergheiANKAVarlous）感染引起的急性致死性综合征 ...更多信息 近交系小鼠对ESM的易感性存在差异（C57 BL/6小鼠高度易感，DBA/2小鼠相对具有抗性）。我们在此报告了使用易感和抗性品系的F（2）杂交群体对DBA/2小鼠中控制ESM抗性的宿主基因组区域进行全基因组扫描的结果。用噬菌体展示技术从疟疾病人血浆中制备了8种IgG和34种IgM，用合成肽分离了4种抗疟抗体（4-5个氨基酸残基）研究了两名患有无并发症或严重恶性疟原虫疟疾的泰国成年患者。无并发症组血清IgE抗体水平最高，而重症组血清总IgE和IgE抗体水平较高。这些结果表明，抗疟原虫IgG抗体参与降低恶性疟原虫疟疾的严重程度，而IgE抗体可能有助于这种感染的发病机制。少

CRI, on erythrocytes shows an Inherited numerical polymorphism which correlates with a HindIII-RFLP of the CRI, gene in Thai patients with acute falciparum malaria for their genotypes of this polymorphism. (ELISA). We observed a significantly higher frequency of homozygotes of the CRI low density allele (LL) among the severe group as compared to the uncomplicated group (P = 0.005). CRI expression on erythrocytes from patients with the LL genotype was significantly lower than homozygotes with the high density allele (HH) (P < 0.0001) and heterozygotes (HL) (P = 0.013). The results suggest that a genetically-determined low CRI density on erythrocytes may be a risk factor for developing a more severe form of malaria in That subjects. Relationship between H frequency and malaria prevalence was examined in Vanuatsu. H frequency was low at islands with low malaria prevalence.Experimental severe malaria (ESM) is an acute lethal syndrome caused by infection with Plasmodium berghei ANKA Varlous … More Inbred strains of mice exhibit differences in susceptibility to the development of ESM (C57BL/6 mice are highly susceptible and DBA/2 mice are relatively resistant) We report here the results of a genomewide scan for host genomic regions that control resistance to ESM in DBA/2 mice using an F(2) intercross population of susceptible and resistant strains. A region of mid-chromosome 18 was found to be a major determinant of resistance to ESM.Eight types of IgG and 34types of IgM were prepared from malaria patients plasma with the use of phage display method and 4 types of antibodies to malaria antigens were isolated with the use of synthetic peptides (4-5 amino acid residues).Two adult Thai patients with either uncomplicated or severe Plasmodium falciparum malaria were studied The mean concentrations of both total IgG and IgG antiplasmodial antibodies tended to be highest in the group with uncomplicated disease while total IgE and IgE antibodies were higher in the group with severe disease. These results suggest that antiplasmodial IgG antibodies are involved in reducing the severity of P. falciparum malaria, while IgE antibodies may contribute to the pathogenesis of this infection. Less