Studies for mechanisms of experimental oral immune-tolerance

实验性口服免疫耐受机制研究

• 批准号: 09460149
• 负责人: YAMADA Takatsugu
• 金额: $ 4.29万
• 依托单位: Azabu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B).
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-09460149/
• 关键词: dog; co-stimulatory signal; CD28; B-7; CD86; genomic DNA; polyclonal antibodies; cDNA; 犬; CD28ペプチド抗体; cDNA library; anergy; スギ花粉; CD4; CD5; CD8; Cry-j; dogs; KLH; 経口免疫寛容

(1) Studies on the effects on canine systemic and local immunity by hemocyanin (KLH) oral administration : CD5 or CD8 positive cells were decreased at 44 days after KLH administration. In KLH administrated group, the mitogen reactions to Con A or KLH were also decreased.(2) The effects on oral immuno-tolerance by Japanese cedar pollen oral administration : CD4 positive cells in BALF cells were increased and the reactions to Cry-j in BALF cells were suppressed in cedar pollen treated group. The reactions to Cry-j in peripheral lymphocytes were also suppressed in treated group.(3) Three types of peptide antibodies for canine CD28 were established by immunizing synthesized peptide to rabbits and prepared IgG or Fab fraction of these antibodies. These antibodies recognized CD28 molecules in lysate of lymphocytes by western blotting. However, they could not recognize CD28 molecules on lymphocyte surface.(4) Canine CD86 cDNA were cloned by Yang. In this experiments, canine CD86 genomic DNA were cloned. The sequences of genomic DNA for canine CD86 were completely same with CD86-mRNA complete code between base number 961-1854. Canine CD86 genomic DNA did not contain intron in this region.

(1)研究口服血青素（KLH）对犬全身和局部免疫的影响：给药后44 d CD5或CD8阳性细胞减少。给药组对Con A和KLH的有丝分裂原反应也降低。(2)口服杉木花粉对口腔免疫耐受的影响：杉木花粉处理组BALF细胞CD4阳性细胞增多，BALF细胞对ry-j的反应受到抑制。治疗组外周淋巴细胞对ry-j的反应也受到抑制。(3)将合成的多肽免疫家兔，制备犬CD28抗体的IgG或Fab片段，建立犬CD28抗体的3种多肽抗体。这些抗体通过免疫印迹法识别淋巴细胞裂解液中的CD28分子。然而，它们不能识别淋巴细胞表面的CD28分子。(4) Yang克隆了犬CD86 cDNA。本实验克隆了犬CD86基因组DNA。犬CD86基因组DNA序列与CD86- mrna全编码序列在961-1854碱基之间完全一致。犬CD86基因组DNA在该区域不含内含子。

项目成果

Yamada, T., Kawano, E., Nishizono, K., Ashida, Y., Shida, T.and Kobayashi, K.: "Evaluation of canine red-blood-cell-bound IgG, IgM, and C3 by mean of a combination of ELISA and the ether-extraction method (in Japanese)"Jpn.J.Vet.Med.Assoc.. 52. 651-857 (1

Yamada, T.、Kawano, E.、Nishizono, K.、Ashida, Y.、Shida, T. 和 Kobayashi, K.：“通过以下方法评估犬红细胞结合的 IgG、IgM 和 C3：

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