Studies for mechanisms of experimental oral immune-tolerance

(1)口服血蓝蛋白（KLH）对犬全身和局部免疫功能影响的研究：KLH给药后44天，CD 5或CD 8阳性细胞减少。KLH组对ConA或KLH的促分裂原反应也明显减弱。(2)日本柳杉花粉对小鼠口服免疫耐受的影响：日本柳杉花粉治疗组BALF细胞中CD_4阳性细胞数增加，对Cry-j的反应受到抑制。治疗组外周血淋巴细胞对Cry-j的反应也受到抑制。(3)用合成的多肽免疫家兔，制备了三种犬CD 28多肽抗体的IgG或Fab片段。这些抗体通过免疫印迹识别淋巴细胞裂解物中的CD 28分子。但不能识别淋巴细胞表面的CD 28分子。(4)Yang等克隆了犬CD 86 cDNA。在本实验中，克隆了犬CD 86基因组DNA。犬CD 86基因组DNA序列与CD 86-mRNA的完整编码序列在961-1854碱基之间完全一致。犬CD 86基因组DNA在该区域不含内含子。

(1) Studies on the effects on canine systemic and local immunity by hemocyanin (KLH) oral administration : CD5 or CD8 positive cells were decreased at 44 days after KLH administration. In KLH administrated group, the mitogen reactions to Con A or KLH were also decreased.(2) The effects on oral immuno-tolerance by Japanese cedar pollen oral administration : CD4 positive cells in BALF cells were increased and the reactions to Cry-j in BALF cells were suppressed in cedar pollen treated group. The reactions to Cry-j in peripheral lymphocytes were also suppressed in treated group.(3) Three types of peptide antibodies for canine CD28 were established by immunizing synthesized peptide to rabbits and prepared IgG or Fab fraction of these antibodies. These antibodies recognized CD28 molecules in lysate of lymphocytes by western blotting. However, they could not recognize CD28 molecules on lymphocyte surface.(4) Canine CD86 cDNA were cloned by Yang. In this experiments, canine CD86 genomic DNA were cloned. The sequences of genomic DNA for canine CD86 were completely same with CD86-mRNA complete code between base number 961-1854. Canine CD86 genomic DNA did not contain intron in this region.