Studies on regenerative medicine by using bone marrow stem cell in intractable hepatic diseases

骨髓干细胞再生医学治疗顽固性肝病的研究

• 批准号: 15580294
• 负责人: YAMADA Takatsugu
• 金额: $ 2.43万
• 依托单位: Azabu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15580294/
• 关键词: bone marrow; canine; c-Met; hepatocyte; HGF; placental extract; regenerative medicine; 幹細胞; 肝臓; C-Met

Next results were obtained by this studies.1) Canine c-Met complete code was determined. c-Met cDNA was showed high homology among that of other animals. c-Met was indicated to be an important factor for canine liver regeneration in experimental partial hepato-ectomy.2) Canine c-Met was suggested to play a role in a part of hematological malignancies, and expected to be a target for the treatment of tumors.3) Canine bone marrow (BM) cells were able to differentiate to hepatocyte-like cells by culture with HGF or human placental extract. In BM cell's differentiating process, HGF was identified as an important factor, and also some other factors in human placental extract were suggested to support HGF functions, (in applying for a patent)4) Further analysis of unknown factors contained in human placental extract would contribute clinical trial of regenerative medicine. The results obtained from this study will be important information not only for veterinary science but also for medical science.

1)确定了犬c-蛋氨酸的完整编码。C-Met基因与其他动物的同源性很高。C-Met是犬实验性肝部分切除后肝再生的重要因素。2)犬c-Met在部分血液系统恶性肿瘤中起一定作用，有望成为治疗肿瘤的靶点。3)犬骨髓细胞经HGF或人胎盘提取液培养后可分化为肝细胞样细胞。在BM细胞的分化过程中，HGF被认为是一个重要的因素，也被认为是人胎盘提取液中支持HGF功能的其他因素(正在申请专利)。4)对人胎盘提取液中未知因素的进一步分析将有助于再生医学的临床试验。这项研究的结果不仅对兽医科学，而且对医学科学都是重要的信息。

项目成果

Alpha-fetoprotein in serum and tumor tissues in dogs with hepatocellular carcinoma

• DOI: 10.1177/104063870601800312
• 发表时间: 2006-05-01
• 期刊: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
• 影响因子: 1.5
• 作者: Kitao, S;Yamada, T;Kobayashi, K
• 通讯作者: Kobayashi, K

Immunoglobulin-A multiple myeloma with hyperecalcemia and pure red-cell aplasia in a cat. (in Japanese)

猫的免疫球蛋白-A 多发性骨髓瘤伴有高钙血症和纯红细胞再生障碍性贫血。

• 发表时间: 2005
• 期刊: J. Jpn. Vet. Med. Assoc. 58
• 作者: Shimoda;T.;Matsukawa;T.;Mizukoshi;T.et al.
• 通讯作者: T.et al.

Treatment of chronic lymphocytic leukaemia in three dogs with melphalan and prednisolone

• DOI: 10.1111/j.1748-5827.2004.tb00239.x
• 发表时间: 2004-06-01
• 期刊: JOURNAL OF SMALL ANIMAL PRACTICE
• 影响因子: 1.6
• 作者: Fujino, Y;Sawamura, S;Tsujimoto, H
• 通讯作者: Tsujimoto, H

Molecular cloning of the canine-c-Met/HGF receptor and its express ion in normal and regenerated liver

犬c-Met/HGF受体的分子克隆及其在正常和再生肝脏中的表达

• 发表时间: 2005
• 期刊: J. Vet. Med. Sci. 67
• 作者: Neo S.;Kanasaku N.;Furuich M.;Watanabe M.;et al.
• 通讯作者: et al.

In vitro selective suppression of feline myeloid colony formation by a molecularly cloned strain of feline leukemia virus with unique long terminal repeat

具有独特长末端重复的猫白血病病毒分子克隆株体外选择性抑制猫骨髓集落形成

• 发表时间: 2005
• 期刊: Res in Vet Sci (in press)
• 作者: Nagashima N;Hisasue M;Nishigaki K;Miyazawa Y;Kano R;Hasegawa
• 通讯作者: Hasegawa