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Cytological studies of the relationship between endocytosymbiosis and arrangement and configuration of organelles in algal cells with special emphasis on the evolutionary significance.

细胞学研究藻类细胞内细胞共生与细胞器排列和构型之间的关系，特别强调进化意义。

基本信息

批准号：
10440245
负责人：
HARA Yoshiaki
金额：
$ 4.35万
依托单位：
Faculty of Science, Yamagata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B).
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 2000
项目状态：
已结题

项目摘要

On the process of evolutionary endosymbiosis, configuration and structure of organelles in the host cell have been turburated by the invation of symbionts. After that, these organelles together with symbiont's ones were rearranged for a novel cell under the genetical and physiological regulation of the host cell. In particular, subcellular diversity found in higher taxa can be considered to be caused by these tuburance and rearrangement of organelles in each cell which was established by endocytosymbiosis. It is not so difficult to assume that these phenomina commonly give rise among phylogenetically different taxa.Using unicellular red algae belonging to the Porphyridiales, Rhodophyta, their subcellular diversity were examined on the focus of their organelles such as chloroplast-pyrenoid and nucleus stained by fluoresent dye under conforcal laser microscope and fluorescent microscopes.As a result, we can distinguished a seies of configurational clain or stepwise morphologies on their … More organellar sturucutres as followings ; 1) photosynthetic organelles increasing their volum and expanding thier surface and a nucleus located not in the center of a cell, 2) embedded pyrenoids changing into naked ones, and chloroplast body showing tendency from central part of cell to the periphery, 3) pyrenoid and nucleus showing the tendency frm cell periphery to the central part of cell, 4) they sometimes compete to occupy the central part of cell with each other, however, nucleus finally occupy the central part of cell, 5) the pyrenoid pushed away from the central part of cell disappearing and chloroplast located cell periphery changing its number in a cell from single to multiple. These results supprted the systematics based on their ultrastructure, habitat, types of the asexual reproduction and composition of phytosynthetic pigments published by Hara and Chihara (1992). In contrast, they could not support the molecular phylogenetic relationship examined by Hara, Yokoyama and Kim (2000) based on their 18SrDNA and psbA Less

在进化的内共生过程中，宿主细胞内细胞器的形态和结构被共生体的侵入所扰乱。然后，在宿主细胞的遗传和生理调控下，这些细胞器与共生体的细胞器一起重新排列形成新的细胞。特别是，在高等类群中发现的亚细胞多样性可以被认为是由这些tuburance和每个细胞中的细胞器的重排所引起的，这些细胞器是通过内吞共生建立的。以红藻门紫球藻目单细胞红藻为材料，在激光共聚焦显微镜和荧光显微镜下观察了它们的叶绿体蛋白核和细胞核等细胞器的结构，结果表明，在不同的藻种中，这些细胞器具有不同的亚细胞结构特征。我们可以在它们的表面上区分出一系列的构型索或阶状形态。 ...更多信息细胞器结构如下; 1）光合细胞器体积增大，表面扩大，核不在细胞中心，2）包埋的蛋白核变为裸露的蛋白核，叶绿体体呈从细胞中心向细胞周边的趋势，3）蛋白核和细胞核呈从细胞周边向细胞中心的趋势，（4）它们有时会竞争占据细胞中央，但最终细胞核占据细胞中央;（5）被挤出细胞中央的蛋白核消失，位于细胞周边的叶绿体由单个变为多个。这些结果支持了Hara和Chihara（1992）根据其超微结构、生境、无性繁殖类型和植物合成色素组成进行的系统分类。相反，它们不能支持Hara，Yokoyama和Kim（2000）基于它们的18 SrDNA和psbA研究的分子系统发育关系。