Studies on the development of functional biopolymers and the removal of pathogenic viruses in the water environment

污水污泥中的大部分病原病毒都是通过活性污泥法去除的。这种对病原病毒的去除主要是由于污泥絮凝体的吸附作用。据说，这种吸附是基于病毒衣壳蛋白与污泥絮凝体中包含的蛋白质之间的相互作用。污水污泥中的蛋白质很有可能被用于去除水环境中的致病病毒。本研究的目的是研究活性污泥中病毒结合蛋白（VBP），并探讨VBP与病原病毒的结合机制。本研究得到以下结论：通过亲和层析技术从活性污泥培养物中分离出vbps。根据分子量（SDS-PAGE估计）将vbp分为两组。它们的分子量分别在11万Da左右和17万Da以上。酶联免疫吸附试验表明，vbp结合脊髓灰质炎病毒1的效率是活性污泥培养粗蛋白的2倍。- vbp的分离点pH分别为7.0和7.7。结果表明，VBPs与病原病毒的结合是基于疏水键和氢键等蛋白之间的相互作用。

Most of pathogenic viruses in the sewage sludge is removed through the activated sludge process. This removal of pathogenic viruses is mainly due to the adsorption to the sludge flocs. It is said that this adsorption is based on the interaction between capsid proteins of viruses and proteins included in the sludge flocs. There is high possibility that the proteins in the sewage sludge can be utilized for the removal of pathogenic viruses from the water environment.The objectives of this research were to survey the virus-binding protein (VBP) from the activeted sludge and to resolve the binding mechanism between the VBP and pathogenic viruses.Following conclusions were obtained in this research :-VBPs were isolated from the activated sludge culture by the affinity chromatography technique.-VBPs were classified into two groups by the molecular weight (estimated by SDS-PAGE). They had the molecular weights around 110,000 Da and over 170,000 Da, respectively.-It became obvious by ELISA method that VBPs could bind poliovirus 1 two times as efficinently as the crude proteins extracted from activated sludge culture.-Isolation points of VBPs were pH 7.0 and 7.7. This result indicated that the binding of pathogenic viruses by VBPs was based on the interaction between proteins such as hydrophobic bond and hydrogen bond.