Development of virus-binding protein-based technologies for concentration, detection and identification of pathogenic viruses in water environment

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The objective of this project was to develop novel technologies for recovery, detection and identification of pathogenic viruses in water environments by taking advantages of our own approaches including virus-binding protein (VBP)and enzymatic virus elution (EVE)method. As a result, enteric virus-binding protein (EVBP)that can capture diverse enteric viruses was successfully identified, and novel methodologies for virus recovery from environmental matrixes such as river and estuarine sediments were established. Furthermore, a novel methodology for detecting infectious pathogenic viruses in water by using ethidium monoazide was also developed. These achievements allow us to complete the original targets of this project.