Challenges to replicate human norovirus with tissue cells

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The main purpose of this project was to acquire precious knowledge for establishing the infection of human norovirus to tissue cells. We successfully constructed a norovirus-like particle with green fluorescence protein (GFP-NoVLP). GFP-NoVLP was applied for confirming the intrusion and decapsidation of human norovirus in tissue cells, but it was found that the amount of fluorescence was not enough to observe the movement of GFP-NoVLP in tissue cells. Therefore, we paid attentions to cellular responses of tissue cells against the virus infection, and tried to identify genetic markers available for the detection of virus infection. Poliovirus type 1 (PV1) Sabin strain was used as a test virus, and a transcriptome analysis of INT407 cells infected with PV1 was conducted. As a result, KCNJ4 gene, responsible for potassium homeostasis, was found as the most promising genetic marker for the detection of virus infection.